@article{Macheroux1987Purif-7105,
  year={1987},
  title={Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore},
  number={1},
  volume={146},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={101--106},
  author={Macheroux, Peter and Schmidt, K. U. and Steinerstauch, Petra and Ghisla, Sandro and Colepicolo, Pio and Buntic, Rudolf and Hastings, J. Woodland}
}

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    <dcterms:issued>1987</dcterms:issued>
    <dc:contributor>Buntic, Rudolf</dc:contributor>
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    <dc:contributor>Schmidt, K. U.</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: Biochemical and Biophysical Research Communications 146 (1987), 1, pp. 101-106</dcterms:bibliographicCitation>
    <dc:language>eng</dc:language>
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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:32Z</dc:date>
    <dc:contributor>Steinerstauch, Petra</dc:contributor>
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    <dc:creator>Buntic, Rudolf</dc:creator>
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    <dc:contributor>Colepicolo, Pio</dc:contributor>
    <dc:creator>Macheroux, Peter</dc:creator>
    <dc:contributor>Macheroux, Peter</dc:contributor>
    <dc:creator>Hastings, J. Woodland</dc:creator>
    <dc:creator>Ghisla, Sandro</dc:creator>
    <dc:creator>Colepicolo, Pio</dc:creator>
    <dc:creator>Schmidt, K. U.</dc:creator>
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    <dc:creator>Steinerstauch, Petra</dc:creator>
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    <dcterms:abstract xml:lang="eng">A low molecular weight protein (~ 25,000 D) exhibiting a yellow fluorescence emission peaking at ~ 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (λmax = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the same strain, which itself catalyzes an emission of blue-green light (λmax ~ 495 nm), this protein induces a bright yellow luminescence (λmax ~ 540 nm); this corresponds to the emission of the Y-1 strain in vivo. This yellow bioluminescence emission is thus ascribed to the interaction of these two proteins, and to the excitation of the singlet FMN bound to this fluorescent protein.</dcterms:abstract>
    <dcterms:title>Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore</dcterms:title>
    <dc:contributor>Hastings, J. Woodland</dc:contributor>
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