@article{Takikawa1986Biosy-7740,
  year={1986},
  doi={10.1111/j.1432-1033.1986.tb10446.x},
  title={Biosynthesis of tetrahydrobiopterin : purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver},
  number={2},
  volume={161},
  issn={0014-2956},
  journal={European Journal of Biochemistry},
  pages={295--302},
  author={Takikawa, Shin-Ichiro and Curtius, Hans-Christoph and Redweik, Udo and Leimbacher, Walter and Ghisla, Sandro}
}

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    <dc:contributor>Ghisla, Sandro</dc:contributor>
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    <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 161 (1986), 2, pp. 295-302</dcterms:bibliographicCitation>
    <dc:creator>Curtius, Hans-Christoph</dc:creator>
    <dc:contributor>Leimbacher, Walter</dc:contributor>
    <dc:contributor>Redweik, Udo</dc:contributor>
    <dcterms:issued>1986</dcterms:issued>
    <dc:creator>Ghisla, Sandro</dc:creator>
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    <dc:contributor>Curtius, Hans-Christoph</dc:contributor>
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    <dc:creator>Redweik, Udo</dc:creator>
    <dcterms:abstract xml:lang="eng">6-Pyruvoyl-tetrahydropterin synthase, which catalyzes the first step in the conversion of 7,8-dihydroneopterin triphosphate to tetrahydrobiopterin, was purified approximately 140,000-fold to apparent homogeneity from human liver. The molecular mass of the enzyme is estimated to be 83 kDa. 7,8-Dihydroneopterin triphosphate was a substrate of the enzyme in the presence of Mg2+, and the pH optimum of the reaction was 7.5 in Tris HCl buffer. The Km value for 7,8-dihydroneopterin triphosphate was 10 μM. The product of this enzymatic reaction was the presumed intermediate 6-pyruvoyl-tetrahydropterin. This latter compound was converted to tetrahydrobiopterin in the presence of NADPH and partially purified sepiapterin reductase from human liver. The conditions and the effect of N-acetyserotonin on this reaction, and on the formation of the intermediates 6-(1'-hydroxy-2'-oxopropyl)-tetrahydropterin and 6-(1'oxo-2'-hydroxypropyl)-tetrahydropterin have been studied.</dcterms:abstract>
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    <dc:contributor>Takikawa, Shin-Ichiro</dc:contributor>
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    <dcterms:title>Biosynthesis of tetrahydrobiopterin : purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver</dcterms:title>
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