Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone

Gallus, Corinna; Schink, Bernhard

Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone

Dateien

Anaerobic_degradation_of_alpha_resorcylate_by_Thauera_aromaticastrain_AR_1_proceeds_via_oxidation_and_decarboxylation_to_hydroxyhydroquinone.pdfGröße: 145.03 KBDownloads: 363

Datum

1998

Autor:innen

Gallus, Corinna

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 1998, 169(4), pp. 333-338. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s002030050579

Zusammenfassung

Anaerobic degradation of alpha-resorcylate (3,5-dihydroxybenzoate) was studied with the denitrifying strain AR-1, which was assigned to the described species Thauera aromatica. Alpha-Resorcylate degradation does not proceed via the benzoyl-CoA, the resorcinol, or the phloroglucinol pathway. Instead, a-resorcylate is converted to hydroxyhydroquinone (1,2,4-trihydroxybenzene) by dehydrogenative oxidation and decarboxylation. Nitrate, K3[Fe(CN)6], dichlorophenol indophenol, and the NAD+ analogue 3-acetylpyridine adeninedinucleotide were suitable electron acceptors for the oxidation reaction; NAD+ did not function as an electron acceptor. The oxidation reaction was strongly accelerated by the additional presence of the redox carrier phenazine methosulfate, which could also be used as sole electron acceptor. Oxidation of alpha-resorcylate with molecular oxygen in cell suspensions or in cell-free extracts of a-resorcylate- and nitrate-grown cells was also detected although this bacterium did not grow with a-resorcylate under an air atmosphere. Alpha-Resorcylate degradation to hydroxyhydroquinone proceeded in two steps. The a-resorcylate-oxidizing enzyme activity was membrane-associated and exhibited maximal activity at pH 8.0. The primary oxidation product was not hydroxyhydroquinone. Rather, formation of hydroxyhydroquinone by decarboxylation of the unknown intermediate in addition required the cytoplasmic fraction and needed lower pH values since hydroxyhydroquinone was not stable at alkaline pH.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

alpha-resorcylate (3,5-dihydroxybenzoate), hydroxyhydroquinone (1,2,4-trihydroxybenzene), aromatic compounds, anaerobic degradation

Zitieren

ISO 690

GALLUS, Corinna, Bernhard SCHINK, 1998. Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone. In: Archives of Microbiology. 1998, 169(4), pp. 333-338. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s002030050579

BibTex

@article{Gallus1998Anaer-8449,
  year={1998},
  doi={10.1007/s002030050579},
  title={Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone},
  number={4},
  volume={169},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={333--338},
  author={Gallus, Corinna and Schink, Bernhard}
}

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