Net synthesis of acetate from CO2 by Eubacterium acidaminophilum through the glycine reductase pathway

Schneeberger, Anne; Frings, Jochen; Schink, Bernhard

Net synthesis of acetate from CO2 by Eubacterium acidaminophilum through the glycine reductase pathway

Dateien

1999_Schneeberger_1_6.pdfGröße: 127.16 KBDownloads: 827

Datum

1999

Autor:innen

Schneeberger, Anne

Frings, Jochen

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEMS Microbiology Letters. 1999, 177(1), pp. 1-6. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1999.tb13705.x

Zusammenfassung

Eubacterium acidaminophilum combines the oxidation of amino acids such as alanine or valine with the reduction of glycine to acetate in a two-substrate fermentation (Stickland reaction). In the absence of glycine, dense cell suspensions oxidized alanine or valine only to a small extent, with limited production of hydrogen and acetate. Experiments with 14C-labeled carbonate revealed that acetate was formed under these conditions by net reduction of CO2/HCO ; 14C-labeled formate was formed as an intermediate. E. acidaminophilum did not grow with hydrogen plus CO2 ; dense cell suspensions under H2/CO2 produced only very small amounts (60.5 mM) of acetate. There was no activity of carbon monoxide dehydrogenase, indicating that the glycine pathway was used for acetate synthesis. The results are explained on the basis of biochemical and energetic considerations.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Glycine reduction, acetogenesis, CO2 fixation, Eubacterium acidaminophilum

Zitieren

ISO 690

SCHNEEBERGER, Anne, Jochen FRINGS, Bernhard SCHINK, 1999. Net synthesis of acetate from CO2 by Eubacterium acidaminophilum through the glycine reductase pathway. In: FEMS Microbiology Letters. 1999, 177(1), pp. 1-6. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1999.tb13705.x

BibTex

@article{Schneeberger1999synth-8772,
  year={1999},
  doi={10.1111/j.1574-6968.1999.tb13705.x},
  title={Net synthesis of acetate from CO2 by Eubacterium acidaminophilum through the glycine reductase pathway},
  number={1},
  volume={177},
  issn={0378-1097},
  journal={FEMS Microbiology Letters},
  pages={1--6},
  author={Schneeberger, Anne and Frings, Jochen and Schink, Bernhard}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8772">
    <dcterms:abstract xml:lang="eng">Eubacterium acidaminophilum combines the oxidation of amino acids such as alanine or valine with the reduction of glycine to acetate in a two-substrate fermentation (Stickland reaction). In the absence of glycine, dense cell suspensions oxidized alanine or valine only to a small extent, with limited production of hydrogen and acetate. Experiments with 14C-labeled carbonate revealed that acetate was formed under these conditions by net reduction of CO2/HCO ; 14C-labeled formate was formed as an intermediate. E. acidaminophilum did not grow with hydrogen plus CO2 ; dense cell suspensions under H2/CO2 produced only very small amounts (60.5 mM) of acetate. There was no activity of carbon monoxide dehydrogenase, indicating that the glycine pathway was used for acetate synthesis. The results are explained on the basis of biochemical and energetic considerations.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:20Z</dc:date>
    <dcterms:issued>1999</dcterms:issued>
    <dcterms:title>Net synthesis of acetate from CO2 by Eubacterium acidaminophilum through the glycine reductase pathway</dcterms:title>
    <dc:creator>Frings, Jochen</dc:creator>
    <dc:contributor>Schneeberger, Anne</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8772/1/1999_Schneeberger_1_6.pdf"/>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:20Z</dcterms:available>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8772"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8772/1/1999_Schneeberger_1_6.pdf"/>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: FEMS Microbiology Letters 177 (1999), pp. 1-6</dcterms:bibliographicCitation>
    <dc:creator>Schneeberger, Anne</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Frings, Jochen</dc:contributor>
    <dc:format>application/pdf</dc:format>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Nein