Anaerobic degradation of 3-hydroxybenzoate by a newly isolated nitrate-reducing bacterium

Heising, Silke; Brune, Andreas; Schink, Bernhard

Anaerobic degradation of 3-hydroxybenzoate by a newly isolated nitrate-reducing bacterium

Dateien

Anaerobic_degradation_of_3_hydroxybenzoate_by_a_newly_isolated_nitrate_reducing_bacterium.pdfGröße: 527.68 KBDownloads: 348

Datum

1991

Autor:innen

Heising, Silke

Brune, Andreas

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEMS Microbiology Letters. 1991, 84(3), pp. 267-272. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1991.tb04608.x

Zusammenfassung

Abstract A gram-negative nitrate-reducing bacterium, strain Asl-3, was isolated from activated sludge with nitrate and 3-hydroxybenzoate as sole source of carbon and energy. The new isolate was faculaatively anaerobic, catalase- and oxidase-positive and polarly monotrichously flagellated. In addition to nitrate, nitrite, N2O, and O2 served as electron acceptors. Growth with 3-hydroxybenzoate and nitrate was biphasic: nitrate was completely reduced to nitrite before nitrite reduction to N2 started. Benzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, protocatechuate or phenyl-acetate served as electron and carbon source under aerobic and anaerobic conditions. During growth with excess carbon source, poly-β-hydroxybutyrate was formed. These characteristics allow the affiliation of strain Asl-3 with the family Pseudomonadaceae. Analogous to the pathway of 4-hydroxybenzoate degradation in other bacteria, the initial step in anaerobic 3-hydroxybenzoate degradation by this organism was activation to 3-hydroxy-benzoyl-CoA in an ATP-consuming reaction. Cell extracts of 3-hydroxybenzoate-grown cells exhibited 3-hydroxybenzoyl-CoA synthetase activity of 190 nmol min−1 mg protein−1 as well as benzoyl-CoA synthetase activity of 86 nmol min−1 mg protein−1. A reductive dehydroxylation of 3-hydroxybenzoyl-CoA could not be demonstrated due to rapid hydrolysis of chemically synthesized 3-hydroxybenzoyl-CoA by cell extracts.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

3-hydroxybenzoyl-CoA synthetase, reductive dehydroxylation, Anaerobic degradation, aromatic compounds, nitrate reduction

Zitieren

ISO 690

HEISING, Silke, Andreas BRUNE, Bernhard SCHINK, 1991. Anaerobic degradation of 3-hydroxybenzoate by a newly isolated nitrate-reducing bacterium. In: FEMS Microbiology Letters. 1991, 84(3), pp. 267-272. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1991.tb04608.x

BibTex

@article{Heising1991Anaer-7126,
  year={1991},
  doi={10.1111/j.1574-6968.1991.tb04608.x},
  title={Anaerobic degradation of 3-hydroxybenzoate by a newly isolated nitrate-reducing bacterium},
  number={3},
  volume={84},
  issn={0378-1097},
  journal={FEMS Microbiology Letters},
  pages={267--272},
  author={Heising, Silke and Brune, Andreas and Schink, Bernhard}
}

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