Effects of alternative methyl group acceptors on the growth energetics of the O-demethylating anaerobe Holophaga foetida

Lade...
Vorschaubild
Datum
1997
Autor:innen
Kappler, Oliver
Janssen, Peter H.
Kreft, Jan-Ulrich
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Microbiology. 1997, 143(4), pp. 1105-1114. ISSN 1350-0872. eISSN 1465-2080. Available under: doi: 10.1099/00221287-143-4-1105
Zusammenfassung

The anaerobic bacterium Holophaga foetida can metabolize the methyl groups of methoxylated aromatic compounds either to acetate or to dimethyl sulphide. The effects of this metabolic flexibility were investigated under conditions of excess substrate (batch culture) and substrate limitation (chemostat culture). Growth yield data suggest that transfer of the methyl groups to sulphide, in contrast to the homoacetogenic transfer to CO2, was not coupled to energy conservation. Under conditions of excess substrate, methyl groups were quantitatively transferred to sulphide. Growth yields decreased but growth rates increased upon the addition of sulphide during exponential growth in pH- and sulphide-regulated batch cultures. From the measured growth yields, the Gibbs free energy dissipation of catabolism plus anabolism (-∆G0'D) was calculated using stoichiometric equations incorporating biomass formation (macrochemical equations). The observed increase in growth rate correlated well with an increase in -∆G0'D, suggesting a relationship between growth kinetics and growth energetics. During steady-state growth in pH- and sulphide-regulated chemostat culture, a considerable fraction of the methyl groups was converted to acetate, despite the presence of sulphide. This resulted in similar growth yields and correspondingly similar -∆G0'D values in the presence and absence of sulphide. Apparently, H. foetida uncouples catabolism and anabolism in batch culture under conditions of excess substrate to a greater extent than in the chemostat under substrate limitation, by transferring the methyl groups quantitatively to sulphide and thereby dissipating the Gibbs free energy change of the methyl transfer. The physiological significance of these findings could be that H. foetida adjusts the energetics of its metabolism to the growth conditions (i) to maximize the growth rate if substrate is available in excess or, (ii) to maximize the growth yield if substrate is limiting.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Holophaga foetida, Gibbs free energy dissipation, O-demethylation, sulphide methylation, syringate
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690KAPPLER, Oliver, Peter H. JANSSEN, Jan-Ulrich KREFT, Bernhard SCHINK, 1997. Effects of alternative methyl group acceptors on the growth energetics of the O-demethylating anaerobe Holophaga foetida. In: Microbiology. 1997, 143(4), pp. 1105-1114. ISSN 1350-0872. eISSN 1465-2080. Available under: doi: 10.1099/00221287-143-4-1105
BibTex
@article{Kappler1997Effec-6647,
  year={1997},
  doi={10.1099/00221287-143-4-1105},
  title={Effects of alternative methyl group acceptors on the growth energetics of the O-demethylating anaerobe Holophaga foetida},
  number={4},
  volume={143},
  issn={1350-0872},
  journal={Microbiology},
  pages={1105--1114},
  author={Kappler, Oliver and Janssen, Peter H. and Kreft, Jan-Ulrich and Schink, Bernhard}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6647">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:03Z</dcterms:available>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dc:creator>Janssen, Peter H.</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Kappler, Oliver</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:title>Effects of alternative methyl group acceptors on the growth energetics of the O-demethylating anaerobe Holophaga foetida</dcterms:title>
    <dcterms:issued>1997</dcterms:issued>
    <dc:creator>Kreft, Jan-Ulrich</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6647/1/Effects_of_alternative_methyl_group_acceptors_on_the_growth_energetics_of_the_O_demethylating_anaerobe_Holophaga_foetida.pdf"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Kreft, Jan-Ulrich</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:abstract xml:lang="eng">The anaerobic bacterium Holophaga foetida can metabolize the methyl groups of methoxylated aromatic compounds either to acetate or to dimethyl sulphide. The effects of this metabolic flexibility were investigated under conditions of excess substrate (batch culture) and substrate limitation (chemostat culture). Growth yield data suggest that transfer of the methyl groups to sulphide, in contrast to the homoacetogenic transfer to CO2, was not coupled to energy conservation. Under conditions of excess substrate, methyl groups were quantitatively transferred to sulphide. Growth yields decreased but growth rates increased upon the addition of sulphide during exponential growth in pH- and sulphide-regulated batch cultures. From the measured growth yields, the Gibbs free energy dissipation of catabolism plus anabolism (-∆G0'D) was calculated using stoichiometric equations incorporating biomass formation (macrochemical equations). The observed increase in growth rate correlated well with an increase in -∆G0'D, suggesting a relationship between growth kinetics and growth energetics. During steady-state growth in pH- and sulphide-regulated chemostat culture, a considerable fraction of the methyl groups was converted to acetate, despite the presence of sulphide. This resulted in similar growth yields and correspondingly similar -∆G0'D values in the presence and absence of sulphide. Apparently, H. foetida uncouples catabolism and anabolism in batch culture under conditions of excess substrate to a greater extent than in the chemostat under substrate limitation, by transferring the methyl groups quantitatively to sulphide and thereby dissipating the Gibbs free energy change of the methyl transfer. The physiological significance of these findings could be that H. foetida adjusts the energetics of its metabolism to the growth conditions (i) to maximize the growth rate if substrate is available in excess or, (ii) to maximize the growth yield if substrate is limiting.</dcterms:abstract>
    <dcterms:bibliographicCitation>First publ. in: Microbiology 143 (1997), pp. 1105-1114</dcterms:bibliographicCitation>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6647"/>
    <dc:contributor>Kappler, Oliver</dc:contributor>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:03Z</dc:date>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6647/1/Effects_of_alternative_methyl_group_acceptors_on_the_growth_energetics_of_the_O_demethylating_anaerobe_Holophaga_foetida.pdf"/>
    <dc:contributor>Janssen, Peter H.</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen