Supplementation with Sterols Improves Food Quality of a Ciliate for Daphnia magna

Martin-Creuzburg, Dominik; Bec, Alexandre; Elert, Eric von

Supplementation with Sterols Improves Food Quality of a Ciliate for Daphnia magna

Dateien

Supplementation_with_Sterols_Improves_Food_Quality_of_a_Ciliate_for_Daphnia_magna.pdfGröße: 114.5 KBDownloads: 496

Datum

2006

Autor:innen

Martin-Creuzburg, Dominik

Bec, Alexandre

Elert, Eric von

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Protist. 2006, 157(4), pp. 477-486. ISSN 1434-4610. Available under: doi: 10.1016/j.protis.2006.07.001

Zusammenfassung

Experimental results provide evidence that trophic interactions between ciliates and Daphnia are constrained by the comparatively low food quality of ciliates. The dietary sterol content is a crucial factor in determining food quality for Daphnia. Ciliates, however, presumably do not synthesize sterols de novo. We hypothesized that ciliates are nutritionally inadequate because of their lack of sterols and tested this hypothesis in growth experiments with Daphnia magna and the ciliate Colpidium campylum. The lipid content of the ciliate was altered by allowing them to feed on fluorescently labeled albumin beads supplemented with different sterols. Ciliates that preyed upon a sterol-free diet (bacteria) did not contain any sterols, and growth of D. magna on these ciliates was poor. Supplementation of the ciliates food source with different sterols led to the incorporation of the supplemented sterols into the ciliates cells and to enhanced somatic growth of D. magna. Sterol limitation was thereby identified as the major constraint of ciliate food quality for Daphnia. Furthermore, by supplementation of sterols unsuitable for supporting Daphnia growth, we provide evidence that ciliates as intermediary grazers biochemically upgrade unsuitable dietary sterols to sterols appropriate to meet the physiological demands of Daphnia.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

albumin beads, cholesterol, Colpidium campylum, dihydrocholesterol, lathosterol, trophic upgrading

Zitieren

ISO 690

MARTIN-CREUZBURG, Dominik, Alexandre BEC, Eric von ELERT, 2006. Supplementation with Sterols Improves Food Quality of a Ciliate for Daphnia magna. In: Protist. 2006, 157(4), pp. 477-486. ISSN 1434-4610. Available under: doi: 10.1016/j.protis.2006.07.001

BibTex

@article{MartinCreuzburg2006Suppl-8528,
  year={2006},
  doi={10.1016/j.protis.2006.07.001},
  title={Supplementation with Sterols Improves Food Quality of a Ciliate for Daphnia magna},
  number={4},
  volume={157},
  issn={1434-4610},
  journal={Protist},
  pages={477--486},
  author={Martin-Creuzburg, Dominik and Bec, Alexandre and Elert, Eric von}
}

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Universitätsbibliographie

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