Pathway of anaerobic poly-beta-hydroxybutyrate degradation by Ilyobacter delafieldii

Janssen, Peter H.; Schink, Bernhard

Pathway of anaerobic poly-beta-hydroxybutyrate degradation by Ilyobacter delafieldii

Dateien

Pathway_of_anaerobic_poly_beta_hydroxybutyrate.pdfGröße: 707.76 KBDownloads: 546

Datum

1993

Autor:innen

Janssen, Peter H.

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Biodegradation. 1993, 4(3), pp. 179-185. ISSN 0923-9820. eISSN 1572-9729. Available under: doi: 10.1007/BF00695120

Zusammenfassung

Ilyobacter delafieldii produced an extracellular poly-[3-hydroxybutyrate (PHB) depolymerase when grown on PHB; activity was not detected in cultures grown on 3-hydroxybutyrate, crotonate, pyruvate or lactate. PHB depolymerase activity was largely associated with the PHB granules (supplied as growth substrate), and only 16 % was detected free in the culture supernatant. Monomeric 3-hydroxybutyrate was detectable as a product of depolymerase activity. The monomer was fermented to acetate, butyrate and H 2. After activation by coen- zyme A transfer from acetyl-CoA or butyryl-CoA, the resultant 3-hydroxybutyryl-CoA was oxidized to ace-toacetyl-CoA (producing NADH), followed by thiolytic cleavage to yield acetyl-CoA which was further me-tabolized to acetyl-phosphate, then to acetate with concomitant ATP production. The reducing equivalents (NADH) could be disposed of by the evolution of H 2, or by a reductive pathway in which 3-hydroxybutyryl-CoA was dehydrated to crotonyl-CoA and reduced to butyryl-CoA. In cocultures of I. delafieldii with Des-ulfovibrio vulgaris on PHB, the H 2 partial pressure was much lower than in the pure cultures, and sulfide was produced. Thus interspecies hydrogen transfer caused a shift to increased acetate and H 2 production at the expense of butyrate.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

PHB, Ilyobacter delafieldii, depolymerase, 3-hydroxybutyrate, extracellular enzyme

Zitieren

ISO 690

JANSSEN, Peter H., Bernhard SCHINK, 1993. Pathway of anaerobic poly-beta-hydroxybutyrate degradation by Ilyobacter delafieldii. In: Biodegradation. 1993, 4(3), pp. 179-185. ISSN 0923-9820. eISSN 1572-9729. Available under: doi: 10.1007/BF00695120

BibTex

@article{Janssen1993Pathw-8572,
  year={1993},
  doi={10.1007/BF00695120},
  title={Pathway of anaerobic poly-beta-hydroxybutyrate degradation by Ilyobacter delafieldii},
  number={3},
  volume={4},
  issn={0923-9820},
  journal={Biodegradation},
  pages={179--185},
  author={Janssen, Peter H. and Schink, Bernhard}
}

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