Batch and continuous production of propionic acid from whey permeate by Propionibacterium acidi-propionici in a three-electrode amperometric culture system

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1992
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Schuppert, Beate
Trösch, Walter
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Applied Microbiology and Biotechnology. 1992, 37(5), pp. 549-553. ISSN 0175-7598. eISSN 1432-0614. Available under: doi: 10.1007/BF00240723
Zusammenfassung

Growth of Propionibacterium acidi-propionici was studied on lactose as substrate and in acid whey permeate in a three-electrode poised-potential system with cobalt sepulchrate as artificial electron donor. In batch culture experiments in a stirred-tank reactor the substrate was fermented completely to propionic acid up to 6.5 g 1 1 lactose in a supplemented whey permeate medium. No acetic acid was produced during the growth of P. acidi-propionici. An electron flow of 80 100 mA was obtained and the electron balance was 101%. In continuously growing cultures with 3 g 1 1 of lactose as the substrate, propionate was formed as the only fermentation product up to a dilution rate (D) of 0.04 h 1. With D>0.04 h 1 the bacteria immobilized on the working electrode surface. It was examined whether an electron transfer occurred between the platinum working electrode and the immobilized cells.

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570 Biowissenschaften, Biologie
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ISO 690SCHUPPERT, Beate, Bernhard SCHINK, Walter TRÖSCH, 1992. Batch and continuous production of propionic acid from whey permeate by Propionibacterium acidi-propionici in a three-electrode amperometric culture system. In: Applied Microbiology and Biotechnology. 1992, 37(5), pp. 549-553. ISSN 0175-7598. eISSN 1432-0614. Available under: doi: 10.1007/BF00240723
BibTex
@article{Schuppert1992Batch-7969,
  year={1992},
  doi={10.1007/BF00240723},
  title={Batch and continuous production of propionic acid from whey permeate by Propionibacterium acidi-propionici in a three-electrode amperometric culture system},
  number={5},
  volume={37},
  issn={0175-7598},
  journal={Applied Microbiology and Biotechnology},
  pages={549--553},
  author={Schuppert, Beate and Schink, Bernhard and Trösch, Walter}
}
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    <dcterms:abstract xml:lang="eng">Growth of Propionibacterium acidi-propionici was studied on lactose as substrate and in acid whey permeate in a three-electrode poised-potential system with cobalt sepulchrate as artificial electron donor. In batch culture experiments in a stirred-tank reactor the substrate was fermented completely to propionic acid up to 6.5 g 1 1 lactose in a supplemented whey permeate medium. No acetic acid was produced during the growth of P. acidi-propionici. An electron flow of 80 100 mA was obtained and the electron balance was 101%. In continuously growing cultures with 3 g 1 1 of lactose as the substrate, propionate was formed as the only fermentation product up to a dilution rate (D) of 0.04 h 1. With D&gt;0.04 h 1 the bacteria immobilized on the working electrode surface. It was examined whether an electron transfer occurred between the platinum working electrode and the immobilized cells.</dcterms:abstract>
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