Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen

Bussmann, Ingeborg; Rahalkar, Monali; Schink, Bernhard

Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen

Dateien

2006_Bussmann_331_344.pdfGröße: 358.72 KBDownloads: 957

Datum

2006

Autor:innen

Bussmann, Ingeborg

Rahalkar, Monali

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEMS Microbiology Ecology. 2006, 56(3), pp. 331-344. ISSN 0168-6496. eISSN 1574-6941. Available under: doi: 10.1111/j.1574-6941.2006.00076.x

Zusammenfassung

In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at a minimum concentration at the layer of consumption. Opposing gradients of methane and oxygen were mimicked in a specific cultivation set-up in which growth of methanotrophic bacteria occurred as a sharp band at either c. 5 or 20mm below the air-exposed end. Two new strains of methanotrophic bacteria were isolated with this system. One isolate, strain LC 1, belonged to the Methylomonas genus (type I methantroph) and contained soluble methane mono-oxygenase. Another isolate, strain LC 2, was related to the Methylobacter group (type I methantroph), as determined by 16S rRNA gene and pmoA sequence similarities. However, the partial pmoA sequence was only 86% related to cultured Methylobacter species. This strain accumulated significant amounts of formaldehyde in conventional cultivation with methane and oxygen, which may explain why it is preferentially enriched in a gradient cultivation system.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Freshwater sediment, gradient cultivation, Lake Constance, methanotrophs, Methylobacter

Zitieren

ISO 690

BUSSMANN, Ingeborg, Monali RAHALKAR, Bernhard SCHINK, 2006. Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen. In: FEMS Microbiology Ecology. 2006, 56(3), pp. 331-344. ISSN 0168-6496. eISSN 1574-6941. Available under: doi: 10.1111/j.1574-6941.2006.00076.x

BibTex

@article{Bussmann2006Culti-8085,
  year={2006},
  doi={10.1111/j.1574-6941.2006.00076.x},
  title={Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygen},
  number={3},
  volume={56},
  issn={0168-6496},
  journal={FEMS Microbiology Ecology},
  pages={331--344},
  author={Bussmann, Ingeborg and Rahalkar, Monali and Schink, Bernhard}
}

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