Studies with Old Yellow Enzyme, Reconstruction with Lumazine Analogs as Coenzyme

Wetzel, Günter; Ghisla, Sandro

Studies with Old Yellow Enzyme, Reconstruction with Lumazine Analogs as Coenzyme

Dateien

Studies_with_Old_Yellow_Enzyme_Reconstruction_with_Lumazine_Analogs_as_Coenzyme1.pdfGröße: 150.08 KBDownloads: 211

Datum

1983

Autor:innen

Wetzel, Günter

Ghisla, Sandro

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-67058

Link zur Lizenz

Attribution-NonCommercial-NoDerivs 2.0 Generic

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Beitrag zu einem Sammelband

Publikationsstatus

Published

Erschienen in

BLAIR, John A., ed.. Chemistry and Biology of Pteridines. Berlin: de Gruyter, 1983, pp. 693-698

Zusammenfassung

8-Substituted lumazines can be regarded as "mutilated" flavins i.e. isoalloxazines lacking the benzene moiety. The 5'phosphorylated, 8-ribityl derivative binds to the apoenzyme of Old Yellow Enzyme with a Kd which is comparable to that of FMN (Kd 6x10-9 M, and 4x10-10 M for the analog, and FMN respectively). Binding induces profound spectral effects on the lumazine chromophore; this might be interpreted as reflecting a protein induced 7alpha-> 1 prototropy. The complex shows approx. 10% of the catalytic activity of native enzyme, in the oxidation of NADPH. Binding of aromatic molecules known to result in pronounced charge transfer spectra with native old yellow enzyme, have no spectral effect with the analog. These results indicate that the benzene moiety is important for the spectral effect, but not for catalysis.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

WETZEL, Günter, Sandro GHISLA, 1983. Studies with Old Yellow Enzyme, Reconstruction with Lumazine Analogs as Coenzyme. In: BLAIR, John A., ed.. Chemistry and Biology of Pteridines. Berlin: de Gruyter, 1983, pp. 693-698

BibTex

@incollection{Wetzel1983Studi-6580,
  year={1983},
  title={Studies with Old Yellow Enzyme, Reconstruction with Lumazine Analogs as Coenzyme},
  publisher={de Gruyter},
  address={Berlin},
  booktitle={Chemistry and Biology of Pteridines},
  pages={693--698},
  editor={Blair, John A.},
  author={Wetzel, Günter and Ghisla, Sandro}
}

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    <dcterms:abstract xml:lang="eng">8-Substituted lumazines can be regarded as "mutilated" flavins i.e. isoalloxazines lacking the benzene moiety. The 5'phosphorylated, 8-ribityl derivative binds to the apoenzyme of Old Yellow Enzyme with a Kd which is comparable to that of FMN (Kd 6x10-9 M, and 4x10-10 M for the analog, and FMN respectively). Binding induces profound spectral effects on the lumazine chromophore; this might be interpreted as reflecting a protein induced 7alpha-&gt; 1 prototropy. The complex shows approx. 10% of the catalytic activity of native enzyme, in the oxidation of NADPH. Binding of aromatic molecules known to result in pronounced charge transfer spectra with native old yellow enzyme, have no spectral effect with the analog. These results indicate that the benzene moiety is important for the spectral effect, but not for catalysis.</dcterms:abstract>
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Universitätsbibliographie

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