Lumazimes as Coenzymes in Flavodoxin : Evidence for the Occurrence of Lumazine Semiquinones

Harzer, Gerd; Ghisla, Sandro

Lumazimes as Coenzymes in Flavodoxin : Evidence for the Occurrence of Lumazine Semiquinones

Dateien

Lumazines_as_Coenzymes_in_Flavodoxin.pdfGröße: 267 KBDownloads: 257

Datum

1979

Autor:innen

Harzer, Gerd

Ghisla, Sandro

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-68251

Link zur Lizenz

Attribution-NonCommercial-NoDerivs 2.0 Generic

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Beitrag zu einem Konferenzband

Publikationsstatus

Published

Erschienen in

Chemistry and Biology of Pteridines : International Symposium Proceedings. Amsterdam: Elsevier, 1979, pp. 37-42

Zusammenfassung

6,7-Dimethyl-N(8)-ribityllumazine-5'-phosphate 1, and 6,7-{2,3-dimethylbutano)-N (8)-ribityllumazine-5'-phosphate 2, bind tightly to apoflavodoxin from Megasphera elsdenii. The complexes are reduced by 0.5 equivalents of dithionite to form the corresponding semiquinones (λmax ~ 460 - 475 nm). An enzyme bound dihydrolumazine species cannot be obtained by direct reduction of the oxidized complexes; 7,8-dihydrolumazine is modified upon binding to apoflavodoxin (λmax ~ 400 nm) suggesting formation of a different tautomer, possibly the S,8-dihydro form. The apoflavodoxin complexes of 1 and 2 show a catalytic activity, ~ 30% and ~ 90% that of the native enzyme in the photosynthetic NADP reduction of spinach chloroplasts. This allows the deduction that electron transfer in flavodoxins does not occur simply via the flavin C(8) position.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

HARZER, Gerd, Sandro GHISLA, 1979. Lumazimes as Coenzymes in Flavodoxin : Evidence for the Occurrence of Lumazine Semiquinones. In: Chemistry and Biology of Pteridines : International Symposium Proceedings. Amsterdam: Elsevier, 1979, pp. 37-42

BibTex

@inproceedings{Harzer1979Lumaz-8397,
  year={1979},
  title={Lumazimes as Coenzymes in Flavodoxin : Evidence for the Occurrence of Lumazine Semiquinones},
  publisher={Elsevier},
  address={Amsterdam},
  booktitle={Chemistry and Biology of Pteridines : International Symposium Proceedings},
  pages={37--42},
  author={Harzer, Gerd and Ghisla, Sandro}
}

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