@article{Galetskiy2008Struc-9766,
  year={2008},
  doi={10.1016/j.jasms.2008.03.014},
  title={Structure and Dynamics of Photosystem II Light-Harvesting Complex Revealed by High-Resolution FTICR Mass Spectrometric Proteome Analysis},
  number={7},
  volume={19},
  issn={1044-0305},
  journal={Journal of the American Society for Mass Spectrometry},
  pages={1004--1013},
  author={Galetskiy, Dmitry and Susnea, Iuliana and Reiser, Verena and Adamska, Iwona and Przybylski, Michael}
}

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    <dcterms:title>Structure and Dynamics of Photosystem II Light-Harvesting Complex Revealed by High-Resolution FTICR Mass Spectrometric Proteome Analysis</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:14:16Z</dc:date>
    <dc:creator>Reiser, Verena</dc:creator>
    <dc:creator>Adamska, Iwona</dc:creator>
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    <dc:contributor>Reiser, Verena</dc:contributor>
    <dc:creator>Przybylski, Michael</dc:creator>
    <dc:contributor>Galetskiy, Dmitry</dc:contributor>
    <dcterms:abstract xml:lang="eng">Structure and dynamics of membrane-bound light-harvesting pigment protein complexes (LHCs), which collect and transmit light energy for photosynthesis and thereby play an essential role in the regulation of photosynthesis and photoprotection, were identified and characterized using high-resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). LHCs from photosystem II (LHCII) were isolated from the thylakoid membrane of Arabidopsis thaliana leaves after light stress treatment using sucrose density gradient centrifugation, and separated by gel-filtration into LHCII subcomplexes. Using reversed-phase high-performance liquid chromatography and two-dimensional (2D) gel electrophoresis, the LHCII proteins, Lhcb1 6 and fibrillins, were efficiently separated and identified by FTICR-MS. Some of the LHCII subcomplexes were shown to migrate from photosystem II to photosystem I as a result of short-term adaptation to changes in light intensity. In the mobile LHCII subcomplexes, decreased levels of fibrillins and a modified composition of LHCII protein isoforms were identified compared to the tightly bound LHCII subcomplexes. In addition, FTICR-MS analysis revealed several oxidative modifications of LHCII proteins. A number of protein spots in 2D gels were found to contain a mixture of proteins, illustrating the feasibility of high-resolution mass spectrometry to identify proteins that remain unseparated in 2D gels even upon extended pH gradients.</dcterms:abstract>
    <dc:contributor>Adamska, Iwona</dc:contributor>
    <dc:contributor>Przybylski, Michael</dc:contributor>
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    <dcterms:bibliographicCitation>First publ. in: Journal of the American Society for Mass Spectrometry 19 (2008), 7, pp. 1004-1013</dcterms:bibliographicCitation>
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    <dcterms:issued>2008</dcterms:issued>
    <dc:contributor>Susnea, Iuliana</dc:contributor>
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