Efficient N-Terminal Glycoconjugation of Proteins by the N-End Rule

Merkel, Lars; Beckmann, Henning S. G.; Wittmann, Valentin; Budisa, Nediljko

Efficient N-Terminal Glycoconjugation of Proteins by the N-End Rule

Dateien

witt_043.pdfGröße: 345.74 KBDownloads: 517

Datum

2008

Autor:innen

Merkel, Lars

Beckmann, Henning S. G.

Wittmann, Valentin

Budisa, Nediljko

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

ChemBioChem. 2008, 9(8), pp. 1220-1224. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.200800050

Zusammenfassung

Bulky amino acids in positions 2 and 3 of proteins protect both Met and noncanonical azidohomoalanine, introduced by the auxotrophy-based method, from being excised by the enzymes responsible for N-terminal Met excision. Bioorthogonal transformation enables new specific functionalization of target proteins. We validated this general concept by designing an N-terminal glycoconjugated barstar capable of lectin binding without losing its biological activity.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

barstar, cycloadditions, glycoproteins, N-end rules, N-terminal methionine excision

Zitieren

ISO 690

MERKEL, Lars, Henning S. G. BECKMANN, Valentin WITTMANN, Nediljko BUDISA, 2008. Efficient N-Terminal Glycoconjugation of Proteins by the N-End Rule. In: ChemBioChem. 2008, 9(8), pp. 1220-1224. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.200800050

BibTex

@article{Merkel2008Effic-9876,
  year={2008},
  doi={10.1002/cbic.200800050},
  title={Efficient N-Terminal Glycoconjugation of Proteins by the N-End Rule},
  number={8},
  volume={9},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={1220--1224},
  author={Merkel, Lars and Beckmann, Henning S. G. and Wittmann, Valentin and Budisa, Nediljko}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/9876">
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9876/1/witt_043.pdf"/>
    <dcterms:issued>2008</dcterms:issued>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:language>eng</dc:language>
    <dcterms:bibliographicCitation>First publ. in: ChemBioChem 9 (2008), 8, pp. 1220-1224</dcterms:bibliographicCitation>
    <dc:contributor>Budisa, Nediljko</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9876/1/witt_043.pdf"/>
    <dc:creator>Merkel, Lars</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:15:01Z</dc:date>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:title>Efficient N-Terminal Glycoconjugation of Proteins by the N-End Rule</dcterms:title>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/9876"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Merkel, Lars</dc:contributor>
    <dc:contributor>Wittmann, Valentin</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:15:01Z</dcterms:available>
    <dc:creator>Beckmann, Henning S. G.</dc:creator>
    <dc:creator>Budisa, Nediljko</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Beckmann, Henning S. G.</dc:contributor>
    <dc:creator>Wittmann, Valentin</dc:creator>
    <dcterms:abstract xml:lang="eng">Bulky amino acids in positions 2 and 3 of proteins protect both Met and noncanonical azidohomoalanine, introduced by the auxotrophy-based method, from being excised by the enzymes responsible for N-terminal Met excision. Bioorthogonal transformation enables new specific functionalization of target proteins. We validated this general concept by designing an N-terminal glycoconjugated barstar capable of lectin binding without losing its biological activity.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja