A new method to measure air-borne pyrogens based on human whole blood cytokine response

Kindinger, Ilona; Daneshian, Mardas; Baur, Hans; Gabrio, Thomas; Hofmann, Andreas; Fennrich, Stefan; Aulock, Sonja von; Hartung, Thomas

A new method to measure air-borne pyrogens based on human whole blood cytokine response

Dateien

2005KindingerJIM_bearb.pdfGröße: 9.2 MBDownloads: 791

Datum

2005

Autor:innen

Kindinger, Ilona

Daneshian, Mardas

Baur, Hans

Gabrio, Thomas

Hofmann, Andreas

Fennrich, Stefan

Aulock, Sonja von

Hartung, Thomas

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of immunological methods. 2005, 298(1-2), pp. 143-153. ISSN 0022-1759. Available under: doi: 10.1016/j.jim.2005.01.006

Zusammenfassung

Air-borne microorganisms, as well as their fragments and components, are increasingly recognized to be associated with pulmonary diseases, e.g. organic dust toxic syndrome, humidifier lung, building-related illness, Monday sickness. We have previously described and validated a new method for the detection of pyrogenic (fever-inducing) microbial contaminations in injectable drugs, based on the inflammatory reaction of human blood to pyrogens. We have now adapted this test to evaluate the total inflammatory capacity of air samples. Air was drawn onto PTFE membrane filters, which were incubated with human whole blood from healthy volunteers inside the collection device. Cytokine release was measured by ELISA. The test detects endotoxins and non-endotoxins, such as fungal spores, Gram-positive bacteria and their lipoteichoic acid moiety and pyrogenic dust particles with high sensitivity, thus reflecting the total inflammatory capacity of a sample. When air from different surroundings such as working environments and animal housing was assayed, the method yielded reproducible data which correlated with other parameters of microbial burden tested. We further developed a standard material for quantification and showed that this assay can be performed with cryopreserved as well as fresh blood. The method offers a test to measure the integral inflammatory capacity of air-borne microbial contaminations relevant to humans. It could thus be employed to assess air quality in different living and work environments.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Air-borne pyrogens, Pyrogen test, Air quality

Zitieren

ISO 690

KINDINGER, Ilona, Mardas DANESHIAN, Hans BAUR, Thomas GABRIO, Andreas HOFMANN, Stefan FENNRICH, Sonja von AULOCK, Thomas HARTUNG, 2005. A new method to measure air-borne pyrogens based on human whole blood cytokine response. In: Journal of immunological methods. 2005, 298(1-2), pp. 143-153. ISSN 0022-1759. Available under: doi: 10.1016/j.jim.2005.01.006

BibTex

@article{Kindinger2005metho-6704,
  year={2005},
  doi={10.1016/j.jim.2005.01.006},
  title={A new method to measure air-borne pyrogens based on human whole blood cytokine response},
  number={1-2},
  volume={298},
  issn={0022-1759},
  journal={Journal of immunological methods},
  pages={143--153},
  author={Kindinger, Ilona and Daneshian, Mardas and Baur, Hans and Gabrio, Thomas and Hofmann, Andreas and Fennrich, Stefan and Aulock, Sonja von and Hartung, Thomas}
}

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Universitätsbibliographie

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