The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown

Göhring, Frank; Schwab, Birgit L.; Nicotera, Pierluigi; Leist, Marcel; Fackelmayer, Frank O.

The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown

Dateien

embo_97.pdfGröße: 584.54 KBDownloads: 286

Datum

1997

Autor:innen

Göhring, Frank

Schwab, Birgit L.

Nicotera, Pierluigi

Leist, Marcel

Fackelmayer, Frank O.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

The EMBO Journal. 1997, 16(24), pp. 7361-7371. ISSN 0261-4189. eISSN 1460-2075. Available under: doi: 10.1093/emboj/16.24.7361

Zusammenfassung

The scaffold attachment factor A (SAF-A) is an abundant component of the nuclear scaffold and of chromatin, and also occurs in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. Evidence from previous experiments had suggested that SAF-A most likely has at least two different functions, being involved both in nuclear architecture and RNA metabolism. We now show that the protein has a novel scaffold-associated region (SAR)-specifi bipartite DNA-binding domain which is independent from the previously identifie RNA-binding domain, the RGG box. During apoptosis, but not during necrosis, SAF-A is cleaved in a caspasedependent way. Cleavage occurs within the bipartite DNA-binding domain, resulting in a loss of DNAbinding activity and a concomitant detachment of SAF-A from nuclear structural sites. On the other hand, cleavage does not compromise the association of SAF-A with hnRNP complexes, indicating that the function of SAF-A in RNA metabolism is not affected in apoptosis. Our results suggest that detachment of SAF-A from SARs, caused by apoptotic proteolysis of its DNA-binding domain, is linked to the formation of oligonucleosomal-sized DNA fragments and could therefore contribute to nuclear breakdown in apoptotic cells.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

GÖHRING, Frank, Birgit L. SCHWAB, Pierluigi NICOTERA, Marcel LEIST, Frank O. FACKELMAYER, 1997. The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown. In: The EMBO Journal. 1997, 16(24), pp. 7361-7371. ISSN 0261-4189. eISSN 1460-2075. Available under: doi: 10.1093/emboj/16.24.7361

BibTex

@article{Gohring1997novel-8454,
  year={1997},
  doi={10.1093/emboj/16.24.7361},
  title={The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown},
  number={24},
  volume={16},
  issn={0261-4189},
  journal={The EMBO Journal},
  pages={7361--7371},
  author={Göhring, Frank and Schwab, Birgit L. and Nicotera, Pierluigi and Leist, Marcel and Fackelmayer, Frank O.}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8454">
    <dc:creator>Fackelmayer, Frank O.</dc:creator>
    <dc:contributor>Göhring, Frank</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8454/1/embo_97.pdf"/>
    <dc:creator>Leist, Marcel</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Leist, Marcel</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:format>application/pdf</dc:format>
    <dc:language>eng</dc:language>
    <dc:contributor>Nicotera, Pierluigi</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8454/1/embo_97.pdf"/>
    <dc:creator>Göhring, Frank</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: The EMBO Journal ; 16 (1997), 24. - pp. 7361-7371</dcterms:bibliographicCitation>
    <dc:creator>Nicotera, Pierluigi</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Fackelmayer, Frank O.</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8454"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Schwab, Birgit L.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Schwab, Birgit L.</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:48Z</dc:date>
    <dcterms:abstract xml:lang="eng">The scaffold attachment factor A (SAF-A) is an abundant component of the nuclear scaffold and of chromatin, and also occurs in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. Evidence from previous experiments had suggested that SAF-A most likely has at least two different functions, being involved both in nuclear architecture and RNA metabolism. We now show that the protein has a novel scaffold-associated region (SAR)-specifi bipartite DNA-binding domain which is independent from the previously identifie RNA-binding domain, the RGG box. During apoptosis, but not during necrosis, SAF-A is cleaved in a caspasedependent way. Cleavage occurs within the bipartite DNA-binding domain, resulting in a loss of DNAbinding activity and a concomitant detachment of SAF-A from nuclear structural sites. On the other hand, cleavage does not compromise the association of SAF-A with hnRNP complexes, indicating that the function of SAF-A in RNA metabolism is not affected in apoptosis. Our results suggest that detachment of SAF-A from SARs, caused by apoptotic proteolysis of its DNA-binding domain, is linked to the formation of oligonucleosomal-sized DNA fragments and could therefore contribute to nuclear breakdown in apoptotic cells.</dcterms:abstract>
    <dcterms:title>The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown</dcterms:title>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:48Z</dcterms:available>
    <dcterms:issued>1997</dcterms:issued>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Nein