Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells

Matentzoglu, Konstantin; Scheffner, Martin

Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells

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Datum

2009

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

BioTechniques. 2009, 46(1), pp. 21-28. ISSN 0736-6205. eISSN 1940-9818. Available under: doi: 10.2144/000113023

Zusammenfassung

Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

MATENTZOGLU, Konstantin, Martin SCHEFFNER, 2009. Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells. In: BioTechniques. 2009, 46(1), pp. 21-28. ISSN 0736-6205. eISSN 1940-9818. Available under: doi: 10.2144/000113023

BibTex

@article{Matentzoglu2009Ubiqu-1271,
  year={2009},
  doi={10.2144/000113023},
  title={Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells},
  number={1},
  volume={46},
  issn={0736-6205},
  journal={BioTechniques},
  pages={21--28},
  author={Matentzoglu, Konstantin and Scheffner, Martin}
}

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Universitätsbibliographie

Ja