- AutorIn
- Sudeshna Chandra
- Christian Gäbler
- Christian Schliebe
- Heinrich Lang
- Dhirendra Bahadur
- Titel
- Fabrication of a label-free electrochemical immunosensor using a redox active ferrocenyl dendrimer
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:ch1-qucosa-220086
- Quellenangabe
- New J. Chem., 2016,40, 9046-9053
- Abstract (DE)
- Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
- Abstract (EN)
- We report an IgG (=immunoglobulin) electrochemical immunosensor using a newly synthesized redox-active ferrocenyl dendrimer of generation 2 (G2Fc) as a voltammetric transducer. The ferrocenyl dendrimer N(CH2CH2C(O)NHCH2CH2NHC(O)Fe(η5-C5H4)(η5-C5H5))(CH2CH2N(CH2CH2C(O)NHCH2CH2NHC(O)Fe(η5-C5H4)(η5-C5H5))2)2 (G2Fc) was used as a functional moiety to immobilize the antibody on the surface of the electrode. A sandwich immunosensor of the type IgG/Bovine serum albumin (BSA)/anti-IgG/G2Fc/glassy carbon electrode (GCE) was fabricated. The electrochemical properties of G2Fc were thoroughly studied in aqueous and non-aqueous electrolytes with varying scan rates. The incubation time was optimized for better analytical performance of the immunosensor. It is found that the developed amperometric immunosensor is sensitive to a concentration of IgG as low as 2 ng mL−1.
- Andere Ausgabe
- DOI: 10.1039/C6NJ00830E
- Link zur Originalpublikation in der Zeitschrift "New Journal of Chemistry"
Link: http://dx.doi.org/10.1039/C6NJ00830E - Freie Schlagwörter (EN)
- immunoglobulin immune-sensor, ferrocene, polyamidoamine dendrimer, spectroelectrochemistry, cancer biomarkers, electrochemical immuno-sensor
- Klassifikation (DDC)
- 540
- Normschlagwörter (GND)
- Ferrocen, Spektroelektrochemie
- Verlag
- Royal Society of Chemistry, Großbritannien
- URN Qucosa
- urn:nbn:de:bsz:ch1-qucosa-220086
- Veröffentlichungsdatum Qucosa
- 06.03.2017
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch