Dokument: The possible treatments for human diseases based on distinct biomechanical properties of living cells utilizing the atomic force microscopy techniques
| Titel: | The possible treatments for human diseases based on distinct biomechanical properties of living cells utilizing the atomic force microscopy techniques | |||||||
| URL für Lesezeichen: | https://docserv.uni-duesseldorf.de/servlets/DocumentServlet?id=56706 | |||||||
| URN (NBN): | urn:nbn:de:hbz:061-20220808-094037-8 | |||||||
| Kollektion: | Dissertationen | |||||||
| Sprache: | Englisch | |||||||
| Dokumententyp: | Wissenschaftliche Abschlussarbeiten » Dissertation | |||||||
| Medientyp: | Text | |||||||
| Autor: | Dr. rer. nat. Tsai, Hsiao-Ching [Autor] | |||||||
| Dateien: |
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| Beitragender: | Prof.Dr M. Getzlaff [Gutachter] | |||||||
| Dewey Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik » 530 Physik | |||||||
| Beschreibung: | Certain diseases are known to cause changes in the biomechanical and biophysical properties of cells. Typically, such biomechanical and biophysical changes can result in several fold increases or decreases in cell elasticity, which are significant, and can result in sever pathology and eventual catastrophic breakdown of the bodingly functions. This study will provide insights into the intercellular structural reorganization, single-cell mechanical characterization of human cells in the context of human diseases.
Force application techniques uses the cell's response of deformation to force applied to the cell as a way to measure cell mechanical properties. Cell functions in response to these stresses are directly related to their mechanical properties. Changes in biomechanics can therefore be correlated with diseases. Single-cell mechanical response characterization and surface-features entails such tools as atomic force microscopy (AFM). AFM represents a developed method to detect the onset or presence of diseases, providing rapid, precise, and sensitive methods to detect and diagnose diseases. The results revealed an approximately two fold increase or decrease in elasticity from normal to diseased cells. Also, the increasing reorganization of the cytoskeleton is observed as compared to normal cells from the onset of disease cells. These diseases include cancer and Progeria. AFM assessment demonstrates the effect of diseases to cellular functions. Details of cellular properties may help to development potential treatments for the human diseases. Applying ultrasound is a widely used technique enabling both diagnostic and therapeutic applications. Different cells can response differently to different frequencies of ultrasound based on their divergence in bio-mechanical properties and morphology. Therefore, characterizing and measuring the properties of cells are essential for the potential treatments. Besides, transfection technique is a procedure artificially introducing foreign nucleic acids (e.g., DNA or RNA) into human cells to produce genetically modified cells. Virus-mediated transfection, the most common used method, deliveries a gene into cells to produce recombinant proteins, or to enhance or inhibit specific gene expression in cells. The function of genes can result in a biomechanical and biophysical changes of cells, and the changes are studied using AFM. | |||||||
| Lizenz: |  Urheberrechtsschutz | |||||||
| Fachbereich / Einrichtung: | Mathematisch- Naturwissenschaftliche Fakultät » WE Physik » Angewandte Physik | |||||||
| Dokument erstellt am: | 08.08.2022 | |||||||
| Dateien geändert am: | 08.08.2022 | |||||||
| Promotionsantrag am: | 28.06.2021 | |||||||
| Datum der Promotion: | 28.06.2021 |
