Universität zu Köln

Hornung, Raphael ORCID: 0000-0002-6729-7289 (2019). Simulation of Growing Cell Sheets. PhD thesis, Universität zu Köln.

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Abstract

Cells grow in sheets in various systems in nature: bacterial films occupy almost every free surface, epithelial cells comprise skin, mucous membranes or glandular tissue, plant cells form leaves and petals. Both in bacteria and body cells, growth is regulated by a combination of biochemical and mechanical cues which are often not understood completely. The response of microscopic cell growth to these various cues is assumed to determine macroscopic behavior of the tissue or cell colony in a self-organized manner. For example, evolution of gut folds during embryonic development is attributed to the reaction of cells to local pressure and growth factor concentration, furthermore, growth behavior of bacterial colonies adapts to the concentration of a variety of diffusing agents such as nutrients, antibiotics or autoinducers. In this work, we study two examples of how local growth regulation mechanisms determine the morphology of cell sheets within an unified simulation framework. First, we investigate wrinkling of a two-dimensional tissue membrane embedded in a dissipative, three-dimensional medium with a local growth rate subject to mechanical feedback. A second research project analyses the growth of monolayered bacterial colonies of the stem Corynebacterium glutamicum in microfluidic devices.

Item Type:	Thesis (PhD thesis)
Creators:	Creators Email ORCID ORCID Put Code Hornung, Raphael raphaelhornung@gmx.de orcid.org/0000-0002-6729-7289 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-103402
Date:	2019
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Außeruniversitäre Forschungseinrichtungen > Forschungszentrum Jülich
Subjects:	Physics Life sciences
Uncontrolled Keywords:	Keywords Language Membranes, Sheets, Growth, Biophysics, Elasticity, Föppl-von Kármán, PIV, Microfluidics, Corynebacterium Glutamicum, Nutrient Limitation English
Date of oral exam:	27 September 2019
Referee:	Name Academic Title Elgeti, Jens Dr. habil. Bollenbach, Tobias Prof. Dr.
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/10340