Universität zu Köln

Guerra, Beatriz A., Brandao, Bruna B., Pinto, Silas S., Salgueiro, Willian G., De-Souza, Evandro A., Reis, Felipe C. G., Batista, Thiago M., Cavalcante-Silva, Vanessa, D'Almeida, Vania ORCID: 0000-0003-0947-5312, Castilho, Beatriz A., Carneiro, Everardo M., Antebi, Adam, Festuccia, William T. and Mori, Marcelo A. (2019). Dietary sulfur amino acid restriction upregulates DICER to confer beneficial effects. Mol. Metab., 29. S. 124 - 136. AMSTERDAM: ELSEVIER. ISSN 2212-8778

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Abstract

Objective: Dietary restriction (DR) improves health and prolongs lifespan in part by upregulating type III endoribonuclease DICER in adipose tissue. In this study, we aimed to specifically test which missing dietary component was responsible for DICER upregulation. Methods: We performed a nutrient screen in mouse preadipocytes and validated the results in vivo using different kinds of dietary interventions in wild type or genetically modified mice and worms, also testing the requirement of DICER on the effects of the diets. Results: We found that sulfur amino acid restriction (i.e., methionine or cysteine) is sufficient to increase Dicer mRNA expression in preadipocytes. Consistently, while DR increases DICER expression in adipose tissue of mice, this effect is blunted by supplementation of the diet with methionine, cysteine, or casein, but not with a lipid or carbohydrate source. Accordingly, dietary methionine or protein restriction mirrors the effects of DR. These changes are associated with alterations in serum adiponectin. We also found that DICER controls and is controlled by adiponectin. In mice, DICER plays a role in methionine restriction-induced upregulation of Ucpl in adipose tissue. In C. elegans, DR and a model of methionine restriction also promote DICER expression in the intestine (an analog of the adipose tissue) and prolong lifespan in a DICER-dependent manner. Conclusions: We propose an evolutionary conserved mechanism in which dietary sulfur amino acid restriction upregulates DICER levels in adipose tissue leading to beneficial health effects. (C) 2019 The Authors. Published by Elsevier GmbH.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Guerra, Beatriz A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brandao, Bruna B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pinto, Silas S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Salgueiro, Willian G. UNSPECIFIED UNSPECIFIED UNSPECIFIED De-Souza, Evandro A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Reis, Felipe C. G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Batista, Thiago M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cavalcante-Silva, Vanessa UNSPECIFIED UNSPECIFIED UNSPECIFIED D'Almeida, Vania UNSPECIFIED orcid.org/0000-0003-0947-5312 UNSPECIFIED Castilho, Beatriz A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Carneiro, Everardo M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Antebi, Adam UNSPECIFIED UNSPECIFIED UNSPECIFIED Festuccia, William T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mori, Marcelo A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-129697
DOI:	10.1016/j.molmet.2019.08.017
Journal or Publication Title:	Mol. Metab.
Volume:	29
Page Range:	S. 124 - 136
Date:	2019
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	2212-8778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADIPOSE-TISSUE INFLAMMATION; METHIONINE RESTRICTION; LIFE-SPAN; CALORIC RESTRICTION; OXIDATIVE STRESS; KINASE GCN2; C. ELEGANS; FAT-CELL; HEALTH; GROWTH Multiple languages Endocrinology & Metabolism Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12969