Universität zu Köln

Lapin, Dmitry ORCID: 0000-0001-9591-3950, Kovacova, Viera, Sun, Xinhua, Dongus, Joram A., Bhandari, Deepak ORCID: 0000-0002-7666-6410, von Born, Patrick, Bautor, Jaqueline, Guarneri, Nina ORCID: 0000-0002-0399-9230, Rzemieniewski, Jakub ORCID: 0000-0001-7274-383X, Stuttmann, Johannes ORCID: 0000-0002-6207-094X, Beyer, Andreas ORCID: 0000-0002-3891-2123 and Parker, Jane E. (2019). A Coevolved EDS1-SAG101-NRG1 Module Mediates Cell Death Signaling by TIR-Domain Immune Receptors. Plant Cell, 31 (10). S. 2430 - 2456. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

Plant nucleotide binding/leucine-rich repeat (NLR) immune receptors are activated by pathogen effectors to trigger host defenses and cell death. Toll-interleukin 1 receptor domain NLRs (TNLs) converge on the ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) family of lipase-like proteins for all resistance outputs. In Arabidopsis (Arabidopsis thaliana) TNL-mediated immunity, AtEDS1 heterodimers with PHYTOALEXIN DEFICIENT4 (AtPAD4) transcriptionally induced basal defenses. AtEDS1 uses the same surface to interact with PAD4-related SENESCENCE-ASSOCIATED GENE101 (AtSAG101), but the role of AtEDS1-AtSAG101 heterodimers remains unclear. We show that AtEDS1-AtSAG101 functions together with N REQUIRED GENE1 (AtNRG1) coiled-coil domain helper NLRs as a coevolved TNL cell death-signaling module. AtEDS1-AtSAG101-AtNRG1 cell death activity is transferable to the Solanaceous species Nicotiana benthamiana and cannot be substituted by AtEDS1-AtPAD4 with AtNRG1 or AtEDS1-AtSAG101 with endogenous NbNRG1. Analysis of EDS1-family evolutionary rate variation and heterodimer structure-guided phenotyping of AtEDS1 variants and AtPAD4-AtSAG101 chimeras identify closely aligned.-helical coil surfaces in the AtEDS1-AtSAG101 partner C-terminal domains that are necessary for reconstituted TNL cell death signaling. Our data suggest that TNL-triggered cell death and pathogen growth restriction are determined by distinctive features of EDS1-SAG101 and EDS1-PAD4 complexes and that these signaling machineries coevolved with other components within plant species or clades to regulate downstream pathways in TNL immunity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lapin, Dmitry UNSPECIFIED orcid.org/0000-0001-9591-3950 UNSPECIFIED Kovacova, Viera UNSPECIFIED UNSPECIFIED UNSPECIFIED Sun, Xinhua UNSPECIFIED UNSPECIFIED UNSPECIFIED Dongus, Joram A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bhandari, Deepak UNSPECIFIED orcid.org/0000-0002-7666-6410 UNSPECIFIED von Born, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED Bautor, Jaqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED Guarneri, Nina UNSPECIFIED orcid.org/0000-0002-0399-9230 UNSPECIFIED Rzemieniewski, Jakub UNSPECIFIED orcid.org/0000-0001-7274-383X UNSPECIFIED Stuttmann, Johannes UNSPECIFIED orcid.org/0000-0002-6207-094X UNSPECIFIED Beyer, Andreas UNSPECIFIED orcid.org/0000-0002-3891-2123 UNSPECIFIED Parker, Jane E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-132738
DOI:	10.1105/tpc.19.00118
Journal or Publication Title:	Plant Cell
Volume:	31
Number:	10
Page Range:	S. 2430 - 2456
Date:	2019
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SALICYLIC-ACID; PHYLOGENETIC ANALYSIS; ARABIDOPSIS EDS1; PLANT; RESISTANCE; PROTEIN; GENES; PAD4; GENOME; RECOGNITION Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13273