Universität zu Köln

Wrobel, Antoni G. ORCID: 0000-0002-6680-5587, Kadlecova, Zuzana, Kamenicky, Jan, Yang, Ji-Chun, Herrmann, Torsten ORCID: 0000-0003-2115-4781, Kelly, Bernard T., Mccoy, Airlie J., Evans, Philip R., Martin, Stephen, Mueller, Stefan, Sroubek, Filip ORCID: 0000-0001-6835-4911, Neuhaus, David, Honing, Stefan and Owen, David J. (2019). Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation. Dev. Cell, 50 (4). S. 494 - 520. CAMBRIDGE: CELL PRESS. ISSN 1878-1551

Full text not available from this repository.

Abstract

Clathrin-mediated endocytosis (CME) is key to maintaining the transmembrane protein composition of cells' limiting membranes. During mammalian CME, a reversible phosphorylation event occurs on Thr156 of the p2 subunit of the main endocytic clathrin adaptor, AP2. We show that this phosphorylation event starts during clathrin-coated pit (CCP) initiation and increases throughout CCP lifetime. mu 2Thr156 phosphorylation favors a new, cargo-bound conformation of AP2 and simultaneously creates a binding platform for the endocytic NECAP proteins but without significantly altering AP2's cargo affinity in vitro. We describe the structural bases of both. NECAP arrival at CCPs parallels that of clathrin and increases with mu 2Thr156 phosphorylation. In turn, NECAP recruits drivers of late stages of CCP formation, including SNX9, via a site distinct from where NECAP binds AP2. Disruption of the different modules of this phosphorylation-based temporal regulatory system results in CCP maturation being delayed and/or stalled, hence impairing global rates of CME.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wrobel, Antoni G. UNSPECIFIED orcid.org/0000-0002-6680-5587 UNSPECIFIED Kadlecova, Zuzana UNSPECIFIED UNSPECIFIED UNSPECIFIED Kamenicky, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Yang, Ji-Chun UNSPECIFIED UNSPECIFIED UNSPECIFIED Herrmann, Torsten UNSPECIFIED orcid.org/0000-0003-2115-4781 UNSPECIFIED Kelly, Bernard T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mccoy, Airlie J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Evans, Philip R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Martin, Stephen UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Sroubek, Filip UNSPECIFIED orcid.org/0000-0001-6835-4911 UNSPECIFIED Neuhaus, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Honing, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Owen, David J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-144143
DOI:	10.1016/j.devcel.2019.07.017
Journal or Publication Title:	Dev. Cell
Volume:	50
Number:	4
Page Range:	S. 494 - 520
Date:	2019
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1878-1551
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NMR STRUCTURE DETERMINATION; TORSION ANGLE DYNAMICS; STRUCTURAL EXPLANATION; NOESY SPECTRA; BINDING; ADAPTER; KINASE; SUBUNIT; DOMAIN; AAK1 Multiple languages Cell Biology; Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14414