Universität zu Köln

Buso, Alessia, Comelli, Marina ORCID: 0000-0002-7620-0805, Picco, Raffaella, Isola, Miriam, Magnesa, Benedetta, Pisot, Rado, Rittweger, Joem, Salvadego, Desy, Simunic, Bostjan, Grassit, Bruno and Mavelli, Irene (2019). Mitochondrial Adaptations in Elderly and Young Men Skeletal Muscle Following 2 Weeks of Bed Rest and Rehabilitation. Front. Physiol., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-042X

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Abstract

The aim of the study was to evaluate the expression levels of proteins related to mitochondrial biogenesis regulation and bioenergetics in vastus lateralis muscle biopsies from 16 elderly and 7 young people subjected to 14 days of bed-rest, causing atrophy, and subsequent 14 days of exercise training. Based on quantitative immunoblot analyses, in both groups a reduction of two key regulators of mitochondrial biogenesis/remodeling and activity, namely PGC-1 alpha and Sirt3, was revealed during bed-rest, with a subsequent up-regulation after rehabilitation, indicating an involvement of PGC-1 alpha-Sirt3 axis in response to the treatments. A difference was observed comparing the young and elderly subjects as, for both proteins, the abundance in the elderly was more affected by immobility and less responsive to exercise. The expression levels of TOM20 and Citrate Synthase, assayed as markers of outer mitochondrial membrane and mitochondrial mass, showed a noticeable sensitivity in the elderly group, where they were affected by bed-rest and rehabilitation recalling the pattern of PGC-1 alpha. TOM20 and CS remained unchanged in young subjects. Single OXPHOS complexes showed peculiar patterns, which were in some cases dissimilar from PGC-1 alpha, and suggest different influences on protein biogenesis and degradation. Overall, exercise was capable to counteract the effect of immobility, when present, except for complex V, which was markedly downregulated by bed-rest, but remained unaffected after rehabilitation, maybe as result of greater extent of degradation processes over biogenesis. Phosphorylation extent of AMPK, and its upstream activator LKB1, did not change after bed-rest and rehabilitation in either young or elderly subjects, suggesting that the activation of energy-sensing LKB1-AMPK signaling pathway was missed due to its transient nature, or was not triggered under our conditions. Our study demonstrates that, as far as the expression of various proteins related to mitochondrial biogenesis/remodeling, adaptations to bed-rest and rehabilitation in the two populations were different. The impact of bed-rest was greater in the elderly subjects, where the pattern (decrease after bed rest and recovery following rehabilitation) was accompanied by changes of mitochondrial mass. Modifications of protein abundance were matched with data obtained from gene expression analyses of four public human datasets focusing on related genes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Buso, Alessia UNSPECIFIED UNSPECIFIED UNSPECIFIED Comelli, Marina UNSPECIFIED orcid.org/0000-0002-7620-0805 UNSPECIFIED Picco, Raffaella UNSPECIFIED UNSPECIFIED UNSPECIFIED Isola, Miriam UNSPECIFIED UNSPECIFIED UNSPECIFIED Magnesa, Benedetta UNSPECIFIED UNSPECIFIED UNSPECIFIED Pisot, Rado UNSPECIFIED UNSPECIFIED UNSPECIFIED Rittweger, Joem UNSPECIFIED UNSPECIFIED UNSPECIFIED Salvadego, Desy UNSPECIFIED UNSPECIFIED UNSPECIFIED Simunic, Bostjan UNSPECIFIED UNSPECIFIED UNSPECIFIED Grassit, Bruno UNSPECIFIED UNSPECIFIED UNSPECIFIED Mavelli, Irene UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-149911
DOI:	10.3389/fphys.2019.00474
Journal or Publication Title:	Front. Physiol.
Volume:	10
Date:	2019
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-042X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MESSENGER-RNA; OXIDATIVE-METABOLISM; DISUSE ATROPHY; GENECHIP DATA; EXERCISE; SIRT3; PROTEIN; ENERGY; PGC-1-ALPHA; EXPRESSION Multiple languages Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14991