Kmietczyk, Vivien, Riechert, Eva ORCID: 0000-0003-4876-7860, Kalinski, Laura, Boileau, Etienne, Malovrh, Ellen ORCID: 0000-0003-0911-6125, Malone, Brandon, Gorska, Agnieszka, Hofmann, Christoph ORCID: 0000-0002-3389-6582, Varma, Eshita, Juergensen, Lonny, Kamuf-Schenk, Verena, Altmueller, Janine, Tappu, Rewati, Busch, Martin, Most, Patrick, Katus, Hugo A., Dieterich, Christoph and Voelkers, Mirko (2019). m(6)A-mRNA methylation regulates cardiac gene expression and cellular growth. Life Sci. Alliance, 2 (2). COLD SPRING HARBOR: LIFE SCIENCE ALLIANCE LLC. ISSN 2575-1077

Full text not available from this repository.

Abstract

Conceptually similar to modifications of DNA, mRNAs undergo chemical modifications, which can affect their activity, localization, and stability. The most prevalent internal modification in mRNA is the methylation of adenosine at the N-6-position (m(6)A). This returns mRNA to a role as a central hub of information within the cell, serving as an information carrier, modifier, and attenuator for many biological processes. Still, the precise role of internal mRNA modifications such as m(6)A in human and murine-dilated cardiac tissue remains unknown. Transcriptome-wide mapping of m(6)A in mRNA allowed us to catalog m(6)A targets in human and murine hearts. Increased m(6)A methylation was found in human cardiomyopathy. Knockdown and overexpression of the m(6)A writer enzyme Mettl3 affected cell size and cellular remodeling both in vitro and in vivo. Our data suggest that mRNA methylation is highly dynamic in cardiomyocytes undergoing stress and that changes in the mRNA methylome regulate translational efficiency by affecting transcript stability. Once elucidated, manipulations of methylation of specific m(6)A sites could be a powerful approach to prevent worsening of cardiac function.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kmietczyk, VivienUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Riechert, EvaUNSPECIFIEDorcid.org/0000-0003-4876-7860UNSPECIFIED
Kalinski, LauraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Boileau, EtienneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Malovrh, EllenUNSPECIFIEDorcid.org/0000-0003-0911-6125UNSPECIFIED
Malone, BrandonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gorska, AgnieszkaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hofmann, ChristophUNSPECIFIEDorcid.org/0000-0002-3389-6582UNSPECIFIED
Varma, EshitaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Juergensen, LonnyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kamuf-Schenk, VerenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Altmueller, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tappu, RewatiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Busch, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Most, PatrickUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Katus, Hugo A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dieterich, ChristophUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Voelkers, MirkoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-151780
DOI: 10.26508/lsa.201800233
Journal or Publication Title: Life Sci. Alliance
Volume: 2
Number: 2
Date: 2019
Publisher: LIFE SCIENCE ALLIANCE LLC
Place of Publication: COLD SPRING HARBOR
ISSN: 2575-1077
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MESSENGER-RNA TRANSLATION; NUCLEAR-RNA; HEART; N-6-METHYLADENOSINE; DIFFERENTIATION; PROTECTS; MUSCLE; MODEL; LINEMultiple languages
BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/15178

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item