Universität zu Köln

Loosen, Sven H., Lurje, Georg, Wiltberger, Georg, Vucur, Mihael, Koch, Alexander, Kather, Jakob N., Paffenholz, Pia, Tacke, Frank, Ulmer, Florian T., Trautwein, Christian, Luedde, Tom, Neumann, Ulf P. and Roderburg, Christoph (2019). Serum levels of miR-29, miR-122, miR-155 and miR-192 are elevated in patients with cholangiocarcinoma. PLoS One, 14 (1). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Objectives Cholangiocarcinoma (CCA) represents the second most common primary hepatic malignancy. Despite tremendous research activities, the prognosis for the majority of patients is still poor. Only in case of early diagnosis, liver resection might potentially lead to long-term survival. However, it is still unclear which patients benefit most from extensive liver surgery, highlighting the need for new diagnostic and prognostic stratification strategies. Methods Serum concentrations of a 4 miRNA panel (miR-122, miR-192, miR-29b and miR-155) were analyzed using semi-quantitative reverse-transcriptase PCR in serum samples from 94 patients with cholangiocarcinoma undergoing tumour resection and 40 healthy controls. Results were correlated with clinical data. Results Serum concentrations of miR-122, miR-192, miR-29b and miR-155 were significantly elevated in patients with CCA compared to healthy controls or patients with primary sclerosing cholangitis without malignant transformation. Although preoperative levels of these miRNAs were unsuitable as a prognostic marker of survival, a strong postoperative decline of miR-122 serum levels was significantly associated with a favorable patients' prognosis. Conclusions Analysis of circulating miRNAs represents a promising tool for the diagnosis of even early stage CCA. A postoperative decline in miRNA serum concentrations might be indicative for a favorable patients' outcome and helpful to identify patients with a good prognosis after extended liver surgery.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Loosen, Sven H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lurje, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiltberger, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Vucur, Mihael UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Kather, Jakob N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Paffenholz, Pia UNSPECIFIED UNSPECIFIED UNSPECIFIED Tacke, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Ulmer, Florian T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Trautwein, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Luedde, Tom UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumann, Ulf P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roderburg, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-159119
DOI:	10.1371/journal.pone.0210944
Journal or Publication Title:	PLoS One
Volume:	14
Number:	1
Date:	2019
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEPATOCELLULAR-CARCINOMA; CIRCULATING MICRORNAS; INTRAHEPATIC CHOLANGIOCARCINOMA; POTENTIAL BIOMARKERS; CANCER; EXPRESSION; DIAGNOSIS; REGULATORS; MORTALITY; MARKERS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15911