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Thomas, Andreas and Thevis, Mario (2019). Recent advances in the determination of insulins from biological fluids. In: Advances in Clinical Chemistry, S. 115 - 168. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC. ISBN 978-0-12-820800-7; 978-0-12-820799-4

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Abstract

The qualitative and quantitative determination of insulin and its related substances (e. g., C-peptide) is of great importance in many different areas of analytical chemistry. In particular, due to the steadily increasing prevalence of metabolic disorders such as diabetes mellitus, an adequate control of the circulating amount of insulin is desirable. In addition, also in forensics and doping control analysis, the determination of insulin in blood, urine or other biological matrices plays a major role. However, in order to establish general reference values for insulin and C-peptide for diabetology, the comparability of measured concentrations is indispensable. This has not yet been fully implemented, although enormous progress has been made in recent years, and the search for a gold standard method is still ongoing. In addition to established ligand-binding assays, an increasing number of mass-spectrometric methods have been developed and employed as the to-date available systems (for example, high-resolution/high accuracy mass spectrometers) provide the sensitivity required to determine analyte concentrations in the sub-ng/mL (sub-100 pmol/L) level. Meanwhile, also high-throughput measurements have been realized to meet the requirement of testing a high number of samples in a short period of time. Further developments aim at enabling the online measurement of insulin in the blood with the help of an insulin sensor and, in the following, in addition to a brief review, today's state of the art testing developments are summarized.

Item Type:	Book Section, Proceedings Item or annotation in a legal commentary
Creators:	Creators Email ORCID ORCID Put Code Thomas, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-159827
DOI:	10.1016/bs.acc.2019.07.003
Title of Book:	Advances in Clinical Chemistry
Series Name:	Advan. Clin. Chem.
Volume:	93
Page Range:	S. 115 - 168
Date:	2019
Publisher:	ELSEVIER ACADEMIC PRESS INC
Place of Publication:	SAN DIEGO
ISSN:	0065-2423
ISBN:	978-0-12-820800-7; 978-0-12-820799-4
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TANDEM MASS-SPECTROMETRY; ISOTOPE-DILUTION ASSAY; GLARGINE HOE 901; GROWTH-FACTOR-I; LIQUID-CHROMATOGRAPHY; C-PEPTIDE; NPH INSULIN; IMMUNOAFFINITY PURIFICATION; CROSS-REACTIVITY; INTACT INSULIN Multiple languages Medical Laboratory Technology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/15982