Universität zu Köln

Kopp, Sascha, Krueger, Marcus ORCID: 0000-0003-2008-4582, Bauer, Johann, Wehland, Markus ORCID: 0000-0002-8160-859X, Corydon, Thomas J., Sahana, Jayashree ORCID: 0000-0002-6606-6315, Nassef, Mohamed Zakaria, Melnik, Daniela, Bauer, Thomas J., Schulz, Herbert, Schuette, Andreas, Schmitz, Burkhard, Oltmann, Hergen, Feldmann, Stefan, Infanger, Manfred and Grimm, Daniela (2018). Microgravity Affects Thyroid Cancer Cells during the TEXUS-53 Mission Stronger than Hypergravity. Int. J. Mol. Sci., 19 (12). BASEL: MDPI. ISSN 1422-0067

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Abstract

Thyroid cancer is the most abundant tumor of the endocrine organs. Poorly differentiated thyroid cancer is still difficult to treat. Human cells exposed to long-term real (r-) and simulated (s-) microgravity (mu g) revealed morphological alterations and changes in the expression profile of genes involved in several biological processes. The objective of this study was to examine the effects of short-term mu g on poorly differentiated follicular thyroid cancer cells (FTC-133 cell line) resulting from 6 min of exposure to mu g on a sounding rocket flight. As sounding rocket flights consist of several flight phases with different acceleration forces, rigorous control experiments are mandatory. Hypergravity (hyper-g) experiments were performed at 18g on a centrifuge in simulation of the rocket launch and s-mu g was simulated by a random positioning machine (RPM). qPCR analyses of selected genes revealed no remarkable expression changes in controls as well as in hyper-g samples taken at the end of the first minute of launch. Using a centrifuge initiating 18g for 1 min, however, presented moderate gene expression changes, which were significant for COL1A1, VCL, CFL1, PTK2, IL6,CXCL8 and MMP14. We also identified a network of mutual interactions of the investigated genes and proteins by employing in-silico analyses. Lastly, mu g-samples indicated that microgravity is a stronger regulator of gene expression than hyper-g.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kopp, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED orcid.org/0000-0003-2008-4582 UNSPECIFIED Bauer, Johann UNSPECIFIED UNSPECIFIED UNSPECIFIED Wehland, Markus UNSPECIFIED orcid.org/0000-0002-8160-859X UNSPECIFIED Corydon, Thomas J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sahana, Jayashree UNSPECIFIED orcid.org/0000-0002-6606-6315 UNSPECIFIED Nassef, Mohamed Zakaria UNSPECIFIED UNSPECIFIED UNSPECIFIED Melnik, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Bauer, Thomas J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulz, Herbert UNSPECIFIED UNSPECIFIED UNSPECIFIED Schuette, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmitz, Burkhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Oltmann, Hergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Feldmann, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Infanger, Manfred UNSPECIFIED UNSPECIFIED UNSPECIFIED Grimm, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-162921
DOI:	10.3390/ijms19124001
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	19
Number:	12
Date:	2018
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SIMULATED MICROGRAVITY; EXTRACELLULAR-MATRIX; 3-DIMENSIONAL GROWTH; ERM PROTEINS; CYTOSKELETON; ADHESION; GRAVITY; BINDING; MOESIN; TALIN Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/16292