Universität zu Köln

Scholz, Philipp, Auler, Markus, Ruthard, Johannes, Brachvogel, Bent, Klatt, Andreas R. and Streichert, Thomas (2018). Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome. Biochem. Medica., 28 (3). ZAGREB: CROATIAN SOC MEDICAL BIOCHEMISTS. ISSN 1846-7482

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Abstract

Introduction: Antibodies specific for annexin A8 (AnxA8) have not been investigated in patients suffering from antiphospholipid syndrome (APS) yet. The aim of this study was to compare the presence of AnxA8 antibodies in serum of APS patients with that of age-matched healthy controls and to investigate whether AnxA8 antibodies are potential biomarkers for APS. Materials and methods: We enrolled 22 APS patients and 22 healthy controls in this case-control study. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblot to investigate the presence of AnxA8 antibodies, and we applied enzyme-linked immunosorbent assay to investigate the presence of cardiolipin (CL) and beta-2-glycoprotein I (beta 2GPI) antibodies. Results: The serum of 9/22 APS patients showed AnxA8 IgG isotype antibody reactivity compared to serum of 2/22 healthy controls (P = 0.034). When we also included weak immunoblot signals, 12/22 APS patients exhibited AnxA8 IgG isotype antibody reactivity compared to 3/22 healthy controls (P = 0.005). We also investigated the presence of AnxA8 IgM isotype antibodies in the serum of APS patients but found no statistically significant difference between the APS patient group and healthy control group (P = 0.500). We further investigated the presence of beta 2GPI and CL IgG and IgM isotype antibodies. AnxA8 IgG isotype antibodies were present in APS patients in a similar frequency as the APS criteria antibody against CL (P = 0.764). Conclusion: We demonstrated that AnxA8 IgG isotype antibodies are potential biomarkers for the diagnosis of APS.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Scholz, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Auler, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruthard, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Brachvogel, Bent UNSPECIFIED UNSPECIFIED UNSPECIFIED Klatt, Andreas R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Streichert, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-170109
DOI:	10.11613/BM.2018.030703
Journal or Publication Title:	Biochem. Medica.
Volume:	28
Number:	3
Date:	2018
Publisher:	CROATIAN SOC MEDICAL BIOCHEMISTS
Place of Publication:	ZAGREB
ISSN:	1846-7482
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION Multiple languages Medical Laboratory Technology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17010