Universität zu Köln

Hemingway, Jordon D., Kusch, Stephanie ORCID: 0000-0002-2708-4975, Walter, Sunita R. Shah, Polik, Catherine A., Elling, Felix J. and Pearson, Ann (2018). A novel method to measure the C-13 composition of intact bacteriohopanepolyols. Org. Geochem., 123. S. 144 - 148. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 0146-6380

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Abstract

We present a novel method to measure the C-13/C-12 isotope ratio (reported as delta C-13) of individual, intact bacteriohopanepolyols (BHPs) using semi-preparative ultrahigh pressure liquid chromatography (UPLC) followed by high temperature gas chromatography-isotope ratio mass spectrometry (HT-GC-IRMS). The method is reproducible, as indicated by the precision of delta C-13 values for bacteriohopanetetrol (BHT) extracted from R. palustris biomass and analyzed across an order-of-magnitude range of IRMS peak areas (delta C-13 = -33.4 +/- 0.6 parts per thousand VPDB, n = 94, +/- 1 sigma). To show that this method successfully separates individual BHPs from complex environmental matrices, we report delta C-13 values for BHT and the BHT stereoisomer (BHT-II) from a ca. 2.9 Ma, organic-rich Mediterranean Sea sediment sample. These analyses suggest that intact BHP delta C-13 measurements can greatly improve the interpretation of environmental signals by alleviating the need for side-chain cleavage which reduces BHP source-specificity. (C) 2018 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hemingway, Jordon D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kusch, Stephanie UNSPECIFIED orcid.org/0000-0002-2708-4975 UNSPECIFIED Walter, Sunita R. Shah UNSPECIFIED UNSPECIFIED UNSPECIFIED Polik, Catherine A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Elling, Felix J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pearson, Ann UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-175732
DOI:	10.1016/j.orggeochem.2018.07.002
Journal or Publication Title:	Org. Geochem.
Volume:	123
Page Range:	S. 144 - 148
Date:	2018
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	0146-6380
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MARINE-SEDIMENTS; BIOMARKERS; HOPANOIDS; BACTERIA; RECORD Multiple languages Geochemistry & Geophysics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17573