Universität zu Köln

Foerstner, Konrad U., Reuscher, Carina M., Haberzettl, Kerstin, Weber, Lennart and Klug, Gabriele ORCID: 0000-0002-3527-5093 (2018). RNase E cleavage shapes the transcriptome of Rhodobacter sphaeroides and strongly impacts phototrophic growth. Life Sci. Alliance, 1 (4). COLD SPRING HARBOR: LIFE SCIENCE ALLIANCE LLC. ISSN 2575-1077

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Abstract

Bacteria adapt to changing environmental conditions by rapid changes in their transcriptome. This is achieved not only by adjusting rates of transcription but also by processing and degradation of RNAs. We applied TIER-Seq (transiently inactivating an endoribonuclease followed by RNA-Seq) for the transcriptome-wide identification of RNase E cleavage sites and of 59 RNA ends, which are enriched when RNase E activity is reduced in Rhodobacter sphaeroides. These results reveal the importance of RNase E for the maturation and turnover of mRNAs, rRNAs, and sRNAs in this guanine-cytosine-rich a-proteobacterium, some of the latter have well-described functions in the oxidative stress response. In agreement with this, a role of RNase E in the oxidative stress response is demonstrated. A remarkably strong phenotype of a mutant with reduced RNase E activity was observed regarding the formation of photosynthetic complexes and phototrophic growth, whereas there was no effect on chemotrophic growth.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Foerstner, Konrad U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Reuscher, Carina M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Haberzettl, Kerstin UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Lennart UNSPECIFIED UNSPECIFIED UNSPECIFIED Klug, Gabriele UNSPECIFIED orcid.org/0000-0002-3527-5093 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-176969
DOI:	10.26508/lsa.201800080
Journal or Publication Title:	Life Sci. Alliance
Volume:	1
Number:	4
Date:	2018
Publisher:	LIFE SCIENCE ALLIANCE LLC
Place of Publication:	COLD SPRING HARBOR
ISSN:	2575-1077
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PUF MESSENGER-RNA; OXIDATIVE-STRESS DEFENSE; IN-VIVO CLEAVAGE; ESCHERICHIA-COLI; SINGLET OXYGEN; GENE-EXPRESSION; RIBONUCLEASE-E; PHOTOSYNTHESIS GENES; CONFERS RESISTANCE; DEGRADATION Multiple languages Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17696