Zhang, Xinxin, Koester, Philipp, Schluecking, Kathrin, Balcerowicz, Daria, Hashimoto, Kenji, Kuchitsu, Kazuyuki ORCID: 0000-0001-8854-0375, Vissenberg, Kris ORCID: 0000-0003-0292-2095 and Kudla, Joerg (2018). CBL1-CIPK26-mediated phosphorylation enhances activity of the NADPH oxidase RBOHC, but is dispensable for root hair growth. FEBS Lett., 592 (15). S. 2582 - 2594. HOBOKEN: WILEY. ISSN 1873-3468

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Abstract

Root hairs (RH) are tip growing polarized cells aiding the uptake of nutrients and water into plants. RH differentiation involves the interplay of various hormones and second messengers. Tightly regulated production of reactive oxygen species by the NADPH oxidase RBOHC crucially functions in RH differentiation and Ca2+-dependent phosphorylation has been implemented in these processes. However, the kinases regulating RBOHC remained enigmatic. Here we identify CBL1-CIPK26 Ca2+ sensor-kinase complexes as modulators of RBOHC activity. Combined genetic, cell biological and biochemical analyses reveal synergistic function of CIPK26-mediated phosphorylation and Ca2+ binding for RBOHC activation. Complementation of rhohC mutant RH phenotypes by a S318/322 phosphorylation deficient RBOHC version suggests flexible and alternating phosphorylation patterns as mechanism fine-tuning ROS production in RH development.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Zhang, XinxinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koester, PhilippUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schluecking, KathrinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Balcerowicz, DariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hashimoto, KenjiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuchitsu, KazuyukiUNSPECIFIEDorcid.org/0000-0001-8854-0375UNSPECIFIED
Vissenberg, KrisUNSPECIFIEDorcid.org/0000-0003-0292-2095UNSPECIFIED
Kudla, JoergUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-177206
DOI: 10.1002/1873-3468.13187
Journal or Publication Title: FEBS Lett.
Volume: 592
Number: 15
Page Range: S. 2582 - 2594
Date: 2018
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1873-3468
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
POSITIVE FEEDBACK-REGULATION; PROTEIN-KINASE; PLANT IMMUNITY; CELL-GROWTH; TIP GROWTH; ARABIDOPSIS; CALCIUM; CA2+; ACTIVATION; REVEALSMultiple languages
Biochemistry & Molecular Biology; Biophysics; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/17720

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