Universität zu Köln

Lores-Motta, Laura ORCID: 0000-0002-2423-9126, Paun, Constantin C., Corominas, Jordi, Pauper, Marc ORCID: 0000-0001-6274-9891, Geerlings, Maartje J., Altay, Lebriz, Schick, Tina, Daha, Mohamed R., Fauser, Sascha, Hoyng, Carel B., den Hollander, Anneke I. and de Jong, Eiko K. (2018). Genome-Wide Association Study Reveals Variants in CFH and CFHR4 Associated with Systemic Complement Activation. Ophthalmology, 125 (7). S. 1064 - 1075. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1549-4713

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Abstract

Purpose: To identify genetic variants associated with complement activation, which may help to select age-related macular degeneration (AMD) patients for complement-inhibiting therapies. Design: Genome-wide association study (GWAS) followed by replication and meta-analysis. Participants: AMD patients and controls (n = 2245). Methods: A GWAS on serum C3d-to-C3 ratio was performed in 1548 AMD patients and controls. For replication and meta-analysis, 697 additional individuals were genotyped. A model for complement activation including genetic and non-genetic factors was built, and the variance explained was estimated. Haplotype analysis was performed for 8 SNPs across the CFH/CFHR locus. Association with AMD was performed for the variants and haplotypes found to influence complement activation. Main Outcome Measures: Normalized C3d/C3 ratio as a measure of systemic complement activation. Results: Complement activation was associated independently with rs3753396 located in CFH (P-discovery 1.09 x 10(-15),; P-meta = 3.66 x 10(-2)1; p = 0.141; standard error [SE] = 0.015) and rs6685931 located in CFHR4 (P-discovery 8.18 x 10(-7),; P-meta= 6.32 x 10-8; p = 0.054; SE = 0.010). A model including age, AMD disease status, body mass index, triglycerides, rs3753396, rs6685931, and previously identified SNPs explained 18.7%of the variability in complement activation. Haplotype analysis revealed 3 haplotypes (H1-2 and H6 containing rs6685931 and H3 containing rs3753396) associated with complement activation. Haplotypes H3 and H6 conferred stronger effects on complement activation compared with the single variants (P = 2.53 x 10(-14); beta= 0.183; SE = 0.024; and P = 4.28 x 10(-4); beta = 0.144; SE = 0.041; respectively). Association analyses with AMD revealed that SNP rs6685931 and haplotype H1-2 containing rs6685931 were associated with a risk for AMD development, whereas SNP rs3753396 and haplotypes H3 and H6 were not. Conclusions: The SNP rs3753396 in CFH and SNP rs6685931 in CFHR4 are associated with systemic complement activation levels. The SNP rs6685931 in CFHR4 and its linked haplotype H1-2 also conferred a risk for AMD development, and therefore could be used to identify AMD patients who would benefit most from complement-inhibiting therapies. (C) 2018 by the American Academy of Ophthalmology.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lores-Motta, Laura UNSPECIFIED orcid.org/0000-0002-2423-9126 UNSPECIFIED Paun, Constantin C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Corominas, Jordi UNSPECIFIED UNSPECIFIED UNSPECIFIED Pauper, Marc UNSPECIFIED orcid.org/0000-0001-6274-9891 UNSPECIFIED Geerlings, Maartje J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Altay, Lebriz UNSPECIFIED UNSPECIFIED UNSPECIFIED Schick, Tina UNSPECIFIED UNSPECIFIED UNSPECIFIED Daha, Mohamed R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fauser, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoyng, Carel B. UNSPECIFIED UNSPECIFIED UNSPECIFIED den Hollander, Anneke I. UNSPECIFIED UNSPECIFIED UNSPECIFIED de Jong, Eiko K. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-180635
DOI:	10.1016/j.ophtha.2017.12.023
Journal or Publication Title:	Ophthalmology
Volume:	125
Number:	7
Page Range:	S. 1064 - 1075
Date:	2018
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1549-4713
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEMOLYTIC-UREMIC SYNDROME; FACTOR-H; MACULAR DEGENERATION; ALTERNATIVE PATHWAY; GENETIC-VARIANTS; AGE; RESISTANCE; PROTEIN; CELLS; RISK Multiple languages Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18063