Schroeder, Daniel C., Maul, Alexandra C., Mahabir, Esther, Koxholt, Isabell, Yan, Xiaowei, Padosch, Stephan A., Herff, Holger, Bultmann-Mellin, Insa, Sterner-Kock, Anja, Annecke, Thorsten ORCID: 0000-0002-2496-4432, Hucho, Tim ORCID: 0000-0002-4147-9308, Boettiger, Bernd W. and Guschlbauer, Maria ORCID: 0000-0002-5401-4542 (2018). Evaluation of small intestinal damage in a rat model of 6 Minutes cardiac arrest. BMC Anesthesiol., 18. LONDON: BIOMED CENTRAL LTD. ISSN 1471-2253

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Abstract

Background: Contribution of the small intestine to systemic inflammation after cardiac arrest (CA) is poorly understood. The objective was to evaluate whether an in vivo rat model of 6 min CA is suitable to initiate intestinal ischaemia-reperfusion-injury and to evaluate histomorphological changes and inflammatory processes in the small intestinal mucosa resp. in sera. Methods: Adult male Wistar rats were subjected to CA followed by cardio-pulmonary resuscitation. Proximal jejunum and serum was collected at 6 h, 24 h, 72 h and 7 d post return of spontaneous circulation (ROSC) and from a control group. The small intestine was evaluated histomorphologically. Cytokine concentrations were measured in jejunum lysates and sera. Results: Histomorphological evaluation revealed a significant increase in mucosal damage in the jejunum at all timepoints compared to controls (p < 0.0001). In jejunal tissues, concentrations of IL-1 alpha, IL-1 beta, IL-10, and TNF-alpha showed significant peaks at 24 h and were 1.5- to 5.7-fold higher than concentrations at 6 h and in the controls (p < 0.05). In serum, a significant higher amount of cytokine was detected only for IL-1 beta at 24 h post-ROSC compared to controls (15.78 vs. 9.76 pg/ml). Conclusion: CA resulted in mild small intestinal tissue damage but not in systemic inflammation. A rat model of 6 min CA is not capable to comprehensively mimic a post cardiac arrest syndrome (PCAS). Whether there is a vital influence of the intestine on the PCAS still remains unclear.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Schroeder, Daniel C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maul, Alexandra C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mahabir, EstherUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koxholt, IsabellUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yan, XiaoweiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Padosch, Stephan A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Herff, HolgerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bultmann-Mellin, InsaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sterner-Kock, AnjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Annecke, ThorstenUNSPECIFIEDorcid.org/0000-0002-2496-4432UNSPECIFIED
Hucho, TimUNSPECIFIEDorcid.org/0000-0002-4147-9308UNSPECIFIED
Boettiger, Bernd W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Guschlbauer, MariaUNSPECIFIEDorcid.org/0000-0002-5401-4542UNSPECIFIED
URN: urn:nbn:de:hbz:38-183036
DOI: 10.1186/s12871-018-0530-8
Journal or Publication Title: BMC Anesthesiol.
Volume: 18
Date: 2018
Publisher: BIOMED CENTRAL LTD
Place of Publication: LONDON
ISSN: 1471-2253
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ISCHEMIA-REPERFUSION-INJURY; JEJUNAL SMOOTH-MUSCLE; CEREBRAL-BLOOD-FLOW; CARDIOPULMONARY-RESUSCITATION; STERILE INFLAMMATION; ANIMAL-MODELS; PATHOPHYSIOLOGY; CONTRACTILITY; DYSFUNCTION; HYPOTHERMIAMultiple languages
AnesthesiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18303

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