Schulz, Alexandra, Fuchs, Paul Christian, Hans, Nadine, Oplaender, Christian, Valdez, Leandra Boerner and Schiefer, Jennifer Lynn ORCID: 0000-0002-3959-9394 (2018). Inhibition of Bromelain Activity During Enzymatic Debridement of Burn Wounds Pretreated With Frequently Used Products. J. Burn Care Res., 39 (3). S. 413 - 423. OXFORD: OXFORD UNIV PRESS. ISSN 1559-0488

Full text not available from this repository.

Abstract

An enzyme mixture containing bromelain (NexoBrid (R)) was found to be suitable for enzymatic debridement of burn wounds, as determined by the criteria of patient comfort and pain, selectivity, and efficiency. Nevertheless, daily experience showed that pretreatment of burn wounds with several other clinical agents may inhibit debridement efficiency. Therefore, the current study was performed to identify those agents and evaluate their debridement inhibition capabilities. The impact of several common agents as well pH, on NexoBrid (R) debridement efficiency was evaluated in vitro. A collagen-based dermal substitute (MatriDerm (R)) was exposed to NexoBrid (R) in the presence of different agents of varying concentrations. Digestion was documented. The criteria used for judging digestion were independently classified by 3 investigators at least 3 times in succession. When a low concentration (1.0 mg/ml) of NexoBrid (R) was used, a >= 50% concentration of Prontosan (R) had an impact on enzymatic activity. Comparable results were obtained when even lower concentrations of Octenisept (R) (>= 10%) were used. A 100-mu mol/L concentration of copper inhibited the enzymatic activity of both a low (1.0 mg/ml) and high (10 mg/ml) concentration of NexoBrid (R). Silver-sulfadiazine at concentrations of 10% and 90% inhibited the activity of 1 mg/ml NexoBrid (R). No complete inhibition of NexoBrid (R) activity occurred at any concentration of iron. We recommend using polyhexanide-containing agents (Prontosan (R)) to rinse and presoak burn wounds. Pretreatment of burn wounds with agents containing silver and copper should be avoided. Experimentally, we found a partial inhibition of NexoBrid (R) activity at the distinct pH values of 3 and 11.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Schulz, AlexandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fuchs, Paul ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hans, NadineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oplaender, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Valdez, Leandra BoernerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schiefer, Jennifer LynnUNSPECIFIEDorcid.org/0000-0002-3959-9394UNSPECIFIED
URN: urn:nbn:de:hbz:38-188474
DOI: 10.1097/BCR.0000000000000609
Journal or Publication Title: J. Burn Care Res.
Volume: 39
Number: 3
Page Range: S. 413 - 423
Date: 2018
Publisher: OXFORD UNIV PRESS
Place of Publication: OXFORD
ISSN: 1559-0488
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CERIUM NITRATE; INJURY; AGENT; MANAGEMENT; EFFICACY; OCTENISEPT(R); POLIHEXANIDE; INFLAMMATION; STABILITY; THERAPYMultiple languages
Critical Care Medicine; Dermatology; SurgeryMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18847

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item