Diesner, Max, Gallot, Aurore, Binz, Hellena, Gaertner, Cyril, Vitecek, Simon ORCID: 0000-0002-7637-563X, Kahnt, Joerg, Schachtner, Joachim ORCID: 0000-0001-7268-0699, Jacquin-Joly, Emmanuelle ORCID: 0000-0002-6904-2036 and Gadenne, Christophe (2018). Mating-Induced Differential Peptidomics of Neuropeptides and Protein Hormones in Agrotis ipsilon Moths. J. Proteome Res., 17 (4). S. 1397 - 1415. WASHINGTON: AMER CHEMICAL SOC. ISSN 1535-3907

Full text not available from this repository.

Abstract

In many insects, mating induces drastic changes in male and female responses to sex pheromones or host-plant odors. In the male moth Agrotis ipsilon, mating induces a transient inhibition of behavioral and neuronal responses to the female sex pheromone. As neuropeptides and peptide hormones regulate most behavioral processes, we hypothesize that they could be involved in this mating-dependent olfactory plasticity. Here we used next-generation RNA sequencing and a combination of liquid chromatography, matrix assisted laser CC desorption ionization time-of-flight (MALDI-TOF) mass and direct tissue profiling to analyze the transcriptome and peptidome of different brain compartments in virgin and mated males and females of A. ipsilon. We identified 37 transcripts encoding putative neuropeptide precursors and 54 putative bioactive neuropeptides from 23 neuropeptide precursors (70 sequences in total, 25 neuropeptide precursors) in different areas of the central nervous system including the antennal lobes, the gnathal ganglion, and the corpora cardiaca-corpora allata complex. Comparisons between virgin and mated males and females revealed tissue-specific differences in peptide composition between sexes and according to physiological state. Mated males showed postmating differences in neuropeptide occurrence, which could participate in the mating-induced olfactory plasticity.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Diesner, MaxUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gallot, AuroreUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Binz, HellenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gaertner, CyrilUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vitecek, SimonUNSPECIFIEDorcid.org/0000-0002-7637-563XUNSPECIFIED
Kahnt, JoergUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schachtner, JoachimUNSPECIFIEDorcid.org/0000-0001-7268-0699UNSPECIFIED
Jacquin-Joly, EmmanuelleUNSPECIFIEDorcid.org/0000-0002-6904-2036UNSPECIFIED
Gadenne, ChristopheUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-190098
DOI: 10.1021/acs.jproteome.7b00779
Journal or Publication Title: J. Proteome Res.
Volume: 17
Number: 4
Page Range: S. 1397 - 1415
Date: 2018
Publisher: AMER CHEMICAL SOC
Place of Publication: WASHINGTON
ISSN: 1535-3907
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ADULT DROSOPHILA-MELANOGASTER; BEETLE TRIBOLIUM-CASTANEUM; SEX-PHEROMONE PRODUCTION; JUVENILE-HORMONE; ANTENNAL LOBES; MANDUCA-SEXTA; SPODOPTERA-LITTORALIS; HELIOTHIS-VIRESCENS; MASS-SPECTROMETRY; NERVOUS-SYSTEMMultiple languages
Biochemical Research MethodsMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/19009

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item