Universität zu Köln

Koloren, Zeynep, Gulabi, Berivan Basak and Karanis, Panagiotis (2018). Molecular identification of Blastocystis sp subtypes in water samples collected from Black sea, Turkey. Acta Trop., 180. S. 58 - 69. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1873-6254

Full text not available from this repository.

Abstract

The aim of this study was to identify the subtypes of Blastocystis sp. and complete a phylogenetic analysis of 268 water samples that were collected from the Samsun, Amasya and Sinop Provinces of the Black Sea in Turkey, between the years 2011 and 2014. Blastocystis sp. was investigated in 48 uncultured sea water samples that were collected from 4 sites within the Sinop Province. A total of 100 river water samples were collected from 37 sites in the Samsun Province and 120 river water samples were collected from 10 sampling sites within the Amasya Province. The small subunit (SSU) rDNA gene Polymerase chain reaction (PCR) were performed for the detection of Blastocytis sp. and the PCR-positive samples were sequenced. Subsequently, the (SSU) rDNA sequences were aligned by Bioedit and phylogenetic trees were constructed for Btastocystis with reference to the genotypes from GenBank. Blastocystis sp. were found in 3 out of the 75 (4%) river water samples that were collected from the Samsun Province. Six of the 120 (5%) river water samples and 1 out of the 48 (2%) seawater samples were positive for Blastocystis in the Amasya and Sinop Provinces. There were two different subtypes (ST; 1 and 3) found from sequencing all of the samples from the investigated 'sites. Two and one PCR products were found to be positive for ST1 and ST3 from the different samples collected within the Samsun Province. Two and 4 PCR products from the Amasya Province were ST1 and ST3, respectively and only one sample from the Sinop Province was found to be positive for ST1. This is the first report to identify and report the occurrence of Blastocystis subtypes within the Black Sea of Turkey.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koloren, Zeynep UNSPECIFIED UNSPECIFIED UNSPECIFIED Gulabi, Berivan Basak UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-192223
DOI:	10.1016/j.actatropica.2017.12.029
Journal or Publication Title:	Acta Trop.
Volume:	180
Page Range:	S. 58 - 69
Date:	2018
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1873-6254
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTESTINAL PARASITES; ZOO ANIMALS; HOMINIS; PREVALENCE; CRYPTOSPORIDIUM; INFECTIONS; EPIDEMIOLOGY; PCR; CLASSIFICATION; PREDOMINANCE Multiple languages Parasitology; Tropical Medicine Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19222