Universität zu Köln

Neupert, Susanne, Marciniak, Pawel ORCID: 0000-0002-4790-001X, Koehler, Rene, Nachman, Ronald J., Suh, Charles P. -C. and Predel, Reinhard (2018). Different processing of CAPA and pyrokinin precursors in the giant mealworm beetle Zophobas atratus (Tenebrionidae) and the boll weevil Anthonomus grandis grandis (Curculionidae). Gen. Comp. Endocrinol., 258. S. 53 - 60. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1095-6840

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Abstract

Capa and pyrokinin (pk) genes in hexapods share a common evolutionary origin. Using transcriptomics and peptidomics, we analyzed products of these genes in two beetles, the giant mealworm beetle (Zophobas atratus; Tenebrionidae) and the boll weevil (Anthonomus grandis grandis; Curculionidae). Our data revealed that even within Coleoptera, which represents a very well-defined group of insects, highly different evolutionary developments occurred in the neuropeptidergic system. These differences, however, primarily affect the general structure of the precursors and differential processing of mature peptides and, to a lesser degree, the sequences of the active core motifs. With the differential processing of the CAPA-precursor in Z. atratus we found a perfect example of completely different products cleaved from a single neuropeptide precursor in different cells. The CAPA precursor in abdominal ganglia of this species yields primarily periviscerokinins (PVKs) whereas processing of the same precursor in neurosecretory cells of the subesophageal ganglion results in CAPA-tryptoPK and a novel CAPA-PK. Particularly important was the detection of that CAPA-PK which has never been observed in the CNS of insects before. The three different types of CAPA peptides (CAPA-tryptoPK, CAPA-PK, PVK) each represent potential ligands which activate different receptors. In contrast to the processing of the CAPA precursor from Z. atratus, no indications of a differential processing of the CAPA precursor were found in A. g. grandis. These data suggest that rapid evolutionary changes regarding the processing of CAPA precursors were still going on when the different beetle lineages diverged. The sequence of the single known PVK of A. g. grandis occupies a special position within the known PVKs of insects and might serve as a basis to develop lineage-specific peptidomimetics capable of disrupting physiological processes regulated by PVKs. (C) 2017 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Neupert, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Marciniak, Pawel UNSPECIFIED orcid.org/0000-0002-4790-001X UNSPECIFIED Koehler, Rene UNSPECIFIED UNSPECIFIED UNSPECIFIED Nachman, Ronald J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Suh, Charles P. -C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-194932
DOI:	10.1016/j.ygcen.2017.08.026
Journal or Publication Title:	Gen. Comp. Endocrinol.
Volume:	258
Page Range:	S. 53 - 60
Date:	2018
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1095-6840
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TOF MASS-SPECTROMETRY; TRIBOLIUM-CASTANEUM; DROSOPHILA-MELANOGASTER; NEUROPEPTIDES; PEPTIDES; RECEPTOR; IDENTIFICATION; PEPTIDOMICS; POPULATIONS; GENOME Multiple languages Endocrinology & Metabolism Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19493