Universität zu Köln

Qureshi, Bilal M., Schmidt, Andrea, Behrmann, Elmar ORCID: 0000-0001-6794-3669, Buerger, Joerg, Mielke, Thorsten, Spahn, Christian M. T., Heck, Martin and Scheerer, Patrick ORCID: 0000-0001-5028-2075 (2018). Mechanistic insights into the role of prenyl-binding protein PrBP/delta in membrane dissociation of phosphodiesterase 6. Nat. Commun., 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

Full text not available from this repository.

Abstract

Isoprenylated proteins are associated with membranes and their inter-compartmental distribution is regulated by solubilization factors, which incorporate lipid moieties in hydrophobic cavities and thereby facilitate free diffusion during trafficking. Here we report the crystal structure of a solubilization factor, the prenyl-binding protein (PrBP/delta), at 1.81 angstrom resolution in its ligand-free apo-form. Apo-PrBP/delta harbors a preshaped, deep hydrophobic cavity, capacitating apo-PrBP/delta to readily bind its prenylated cargo. To investigate the molecular mechanism of cargo solubilization we analyzed the PrBP/delta-induced membrane dissociation of rod photoreceptor phosphodiesterase (PDE6). The results suggest that PrBP/delta exclusively interacts with the soluble fraction of PDE6. Depletion of soluble species in turn leads to dissociation of membrane-bound PDE6, as both are in equilibrium. This solubilization by depletion mechanism of PrBP/delta differs from the extraction of prenylated proteins by the similar folded solubilization factor RhoGDI, which interacts with membrane bound cargo via an N-terminal structural element lacking in PrBP/delta.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Qureshi, Bilal M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Behrmann, Elmar UNSPECIFIED orcid.org/0000-0001-6794-3669 UNSPECIFIED Buerger, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Mielke, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Spahn, Christian M. T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Heck, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheerer, Patrick UNSPECIFIED orcid.org/0000-0001-5028-2075 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-199052
DOI:	10.1038/s41467-017-02569-y
Journal or Publication Title:	Nat. Commun.
Volume:	9
Date:	2018
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ROD CGMP PHOSPHODIESTERASE; PDE-DELTA; INHIBITORY SUBUNIT; TRANSPORT; TRAFFICKING; SOLUBILIZATION; TRANSDUCIN; EFFECTOR; COMPLEX; DOMAIN Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19905