Universität zu Köln

Piatkowski, Tanja, Brandenberger, Christina, Rahmanian, Parwis, Choi, Yeong-Hoon, Zeriouh, Mohamed, Sabashnikov, Anton, Wittwer, Thorsten, Wahlers, Thorsten C. W., Ochs, Matthias and Muehlfeld, Christian (2018). Localization of Exogenous Mesenchymal Stem Cells in a Pig Model of Lung Transplantation. Thorac. Cardiovasc. Surg., 66 (1). S. 63 - 71. STUTTGART: GEORG THIEME VERLAG KG. ISSN 1439-1902

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Abstract

Background Mesenchymal stem cells (MSCs) have a great potential for the treatment of acute lung injury. This study provides a detailed immunohistochemical and stereological analysis of the localization and distribution of exogenous MSC in a pig model of lung transplantation after intravascular or endobronchial application. Methods MSC derived from human bone marrow were labeled by DiI and administered intravascularly or endobronchially to the lungs of donor pigs after a period of 3 hours warm and 3 hours cold ischemia. The left lung was transplanted to a recipient pig and reperfused for 4 hours before fixation. The right donor lung was fixed for microscopic analysis directly after the ischemia time. Results After both administration routes, a similar number of exogenous MSC was found in the lungs. Within each animal, the heterogeneity of MSC distribution was high both with respect to left and right lung as well as to the different lobes of each lung. After endobronchial application, MSC were found in alveolar and bronchial/bronchiolar lumen, whereas after intravascular administration, they were mainly observed in blood vessels. Conclusion Although the administration of exogenous MSC is possible by endobronchial or intravascular application, it yields a heterogeneous distribution in the lungs which may warrant strategies to improve a more homogeneous distribution.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Piatkowski, Tanja UNSPECIFIED UNSPECIFIED UNSPECIFIED Brandenberger, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahmanian, Parwis UNSPECIFIED UNSPECIFIED UNSPECIFIED Choi, Yeong-Hoon UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeriouh, Mohamed UNSPECIFIED UNSPECIFIED UNSPECIFIED Sabashnikov, Anton UNSPECIFIED UNSPECIFIED UNSPECIFIED Wittwer, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Wahlers, Thorsten C. W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ochs, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Muehlfeld, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-204579
DOI:	10.1055/s-0037-1607051
Journal or Publication Title:	Thorac. Cardiovasc. Surg.
Volume:	66
Number:	1
Page Range:	S. 63 - 71
Date:	2018
Publisher:	GEORG THIEME VERLAG KG
Place of Publication:	STUTTGART
ISSN:	1439-1902
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ISCHEMIA-REPERFUSION INJURY; ALVEOLAR FLUID CLEARANCE; STROMAL CELLS; ENGRAFTMENT; SURFACTANT; PNEUMONIA; INFUSION; PROMOTES; POSITION Multiple languages Cardiac & Cardiovascular Systems; Respiratory System; Surgery Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20457