Universität zu Köln

Poomvanicha, Montatip, Matthes, Jan ORCID: 0000-0003-2754-1555, Domes, Katrin, Patrucco, Enrico ORCID: 0000-0001-8060-5058, Angermeier, Elisabeth, Laugwitz, Karl-Ludwig, Schneider, Toni and Hofmann, Franz (2017). Beta-adrenergic regulation of the heart expressing the Serl 700A/Thr1704A mutated Cav1.2 channel. J. Mol. Cell. Cardiol., 111. S. 10 - 17. OXFORD: ELSEVIER SCI LTD. ISSN 1095-8584

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Abstract

Beta-adrenergic stimulation of the heart increases l(Ca). PRA dependent phosphorylation of several amino acids (among them Ser 1700 and Thr 1704 in the carboxy-terminus of the Cav1.2 alpha(1)c subunit) has been implicated as decisive for the beta-adrenergic up-regulation of cardiac I-ca. Mutation of Ser 1700 and Thr 1704 to alanine results in the Cav1.2PKA.92(-/-) mice. Cav1.2PKA_P2(-/-) mice display reduced cardiac L-type current. Fractional shortening and ejection fraction in the intact animal and la, in isolated cardiomyocytes (CM) are stimulated by isoproterenol. Cardiac specific expression of the mutated Cav1.2PKA_P2(-/-) gene reduces Cav1.2 laic protein concentration, lab and the beta-adrenergic stimulation of L-type l(Ca), in CMs. Single channels were not detected on the CM surface of the cCav1.2PKA_P2(-/-) hearts. This outcome supports the notion that S1700/1704 is essential for expression of the Cav1.2 channel and that isoproterenol stimulates la, in Cav1.2PIOU32(-/-) CMs. (C) 2017 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Poomvanicha, Montatip UNSPECIFIED UNSPECIFIED UNSPECIFIED Matthes, Jan UNSPECIFIED orcid.org/0000-0003-2754-1555 UNSPECIFIED Domes, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Patrucco, Enrico UNSPECIFIED orcid.org/0000-0001-8060-5058 UNSPECIFIED Angermeier, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Laugwitz, Karl-Ludwig UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Toni UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Franz UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-215668
DOI:	10.1016/j.yjmcc.2017.07.119
Journal or Publication Title:	J. Mol. Cell. Cardiol.
Volume:	111
Page Range:	S. 10 - 17
Date:	2017
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	1095-8584
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DEPENDENT PROTEIN-KINASE; CALCIUM-CHANNELS; CA2+ CHANNEL; PHOSPHORYLATION SITES; CA(V)1.2; MODULATION; SUBUNIT; MICE; FAILURE; CARDIOMYOCYTES Multiple languages Cardiac & Cardiovascular Systems; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21566