Huelsemann, Frank, Koehler, Karsten ORCID: 0000-0002-9618-2069, Flenker, Ulrich and Schaenzer, Wilhelm (2017). Do we excrete what we eat? Analysis of stable nitrogen isotope ratios of human urinary urea. Rapid Commun. Mass Spectrom., 31 (14). S. 1221 - 1228. HOBOKEN: WILEY. ISSN 1097-0231

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Abstract

RATIONALE: Natural stable nitrogen isotope ratios (delta N-15) are frequently used for the determination of provenance and dietary assessment of recent and ancient humans. Although individual delta N-15 values typically correspond to the dietary delta N-15 composition, they are also affected by metabolic conditions. Preferred matrices for the measurement of human delta N-15 values have been hair, nail or blood. The goal of this study was to validate a novel approach for the assessment of the delta N-15 values from urinary urea, the principal end-product of human N metabolism. METHODS: The method, which involves the precipitation of urea from urine using xanthydrol, was validated using fortified urea solutions. Intra-and inter-individual variance of the delta N-15 values of urinary urea was determined from samples obtained from multiple human subjects. RESULTS: Precipitation with xanthydrol did not alter the delta N-15 values of urea. The mean delta N-15 value in urinary urea from human subjects from Germany was +4.4 +/- 0.6%, which corresponds to the estimated dietary composition. It falls below previously reported delta N-15 values for human tissue and blood samples. Longitudinal analyses over 7 days illustrate short-time changes linked to varying protein intake. CONCLUSIONS: Our results indicate that delta N-15 values can be measured reliably from human urine and that the method is suitable to monitor rapid dietary and metabolic changes of an individual. Our findings further confirm that urinary urea is depleted in N-15 compared with human tissue but within the range of the delta N-15 composition of the diet. Copyright (C) 2017 John Wiley & Sons, Ltd.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Huelsemann, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koehler, KarstenUNSPECIFIEDorcid.org/0000-0002-9618-2069UNSPECIFIED
Flenker, UlrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schaenzer, WilhelmUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-224602
DOI: 10.1002/rcm.7891
Journal or Publication Title: Rapid Commun. Mass Spectrom.
Volume: 31
Number: 14
Page Range: S. 1221 - 1228
Date: 2017
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1097-0231
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MODERN HUMAN HAIR; CONTROLLED DIETARY; RANGIFER-TARANDUS; PROTEIN STATUS; N-15; C-13; BALANCE; CARIBOU; CARBON; DELTA-N-15Multiple languages
Biochemical Research Methods; Chemistry, Analytical; SpectroscopyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/22460

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