Wawra, Stephan ORCID: 0000-0001-8555-1618, Trusch, Franziska ORCID: 0000-0003-0608-202X, Matena, Anja, Apostolakis, Kostis, Linne, Uwe, Zhukov, Igor, Stanek, Jan ORCID: 0000-0003-1660-168X, Kozminski, Wiktor ORCID: 0000-0003-2319-4525, Davidson, Ian, Secombes, Chris J., Bayer, Peter ORCID: 0000-0003-0435-7202 and van Westa, Pieter (2017). The RxLR Motif of the Host Targeting Effector AVR3a of Phytophthora infestans Is Cleaved before Secretion. Plant Cell, 29 (6). S. 1184 - 1196. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1531-298X

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Abstract

When plant-pathogenic oomycetes infect their hosts, they employ a large arsenal of effector proteins to establish a successful infection. Some effector proteins are secreted and are destined to be translocated and function inside host cells. The largest group of translocated proteins from oomycetes is the RxLR effectors, defined by their conserved N-terminal Arg-Xaa-Leu-Arg (RxLR) motif. However, the precise role of this motif in the host cell translocation process is unclear. Here, detailed biochemical studies of the RxLR effector AVR3a from the potato pathogen Phytophthora infestans are presented. Mass spectrometric analysis revealed that the RxLR sequence of native AVR3a is cleaved off prior to secretion by the pathogen and the N terminus of the mature effector was found likely to be acetylated. High-resolution NMR structure analysis of AVR3a indicates that the RxLR motif is well accessible to potential processing enzymes. Processing and modification of AVR3a is to some extent similar to events occurring with the export element (PEXEL) found in malaria effector proteins from Plasmodium falciparum. These findings imply a role for the RxLR motif in the secretion of AVR3a by the pathogen, rather than a direct role in the host cell entry process itself.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Wawra, StephanUNSPECIFIEDorcid.org/0000-0001-8555-1618UNSPECIFIED
Trusch, FranziskaUNSPECIFIEDorcid.org/0000-0003-0608-202XUNSPECIFIED
Matena, AnjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Apostolakis, KostisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Linne, UweUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhukov, IgorUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stanek, JanUNSPECIFIEDorcid.org/0000-0003-1660-168XUNSPECIFIED
Kozminski, WiktorUNSPECIFIEDorcid.org/0000-0003-2319-4525UNSPECIFIED
Davidson, IanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Secombes, Chris J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bayer, PeterUNSPECIFIEDorcid.org/0000-0003-0435-7202UNSPECIFIED
van Westa, PieterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-230186
DOI: 10.1105/tpc.16.00552
Journal or Publication Title: Plant Cell
Volume: 29
Number: 6
Page Range: S. 1184 - 1196
Date: 2017
Publisher: AMER SOC PLANT BIOLOGISTS
Place of Publication: ROCKVILLE
ISSN: 1531-298X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PHOSPHATIDYLINOSITOL 3-PHOSPHATE; HYALOPERONOSPORA-ARABIDOPSIDIS; MALARIA PROTEINS; STRUCTURAL BASIS; PLASMA-MEMBRANE; CELL-DEATH; PLANT; OOMYCETE; EXPORT; VIRULENCEMultiple languages
Biochemistry & Molecular Biology; Plant Sciences; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/23018

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