Universität zu Köln

Diegelmann, Soeren, Jansen, Annika, Jois, Shreyas, Kastenholz, Katharina, Escarcena, Laura Velo, Strudthoff, Nicole and Scholz, Henrike (2017). The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster. J. Vis. Exp. (121). CAMBRIDGE: JOURNAL OF VISUALIZED EXPERIMENTS. ISSN 1940-087X

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Abstract

For most animals, feeding is an essential behavior for securing survival, and it influences development, locomotion, health and reproduction. Ingestion of the right type and quantity of food therefore has a major influence on quality of life. Research on feeding behavior focuses on the underlying processes that ensure actual feeding and unravels the role of factors regulating internal energy homeostasis and the neuronal bases of decision-making. The model organism Drosophila melanogaster, with its great variety of genetically traceable tools for labeling and manipulating single neurons, allows mapping of neuronal networks and identification of molecular signaling cascades involved in the regulation of food intake. This report demonstrates the CApillary FEeder assay (CAFE) and shows how to measure food intake in a group of flies for time spans ranging from hours to days. This easy-to-use assay consists of glass capillaries filled with liquid food that flies can freely access and feed on. Food consumption in the assay is accurately determined using simple measurement tools. Herein we describe step-by-step the method from setup to successful execution of the CAFE assay, and provide practical examples to analyze the food intake of a group of flies under controlled conditions. The reader is guided through possible limitations of the assay, and advantages and disadvantages of the method compared to other feeding assays in D. melanogaster are evaluated.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Diegelmann, Soeren UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansen, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Jois, Shreyas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kastenholz, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Escarcena, Laura Velo UNSPECIFIED UNSPECIFIED UNSPECIFIED Strudthoff, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Scholz, Henrike UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-237696
DOI:	10.3791/55024
Journal or Publication Title:	J. Vis. Exp.
Number:	121
Date:	2017
Publisher:	JOURNAL OF VISUALIZED EXPERIMENTS
Place of Publication:	CAMBRIDGE
ISSN:	1940-087X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADULT DROSOPHILA; FEEDING-BEHAVIOR; FRUIT-FLIES; METABOLISM; QUANTIFICATION; SEROTONIN; INSIGHTS; OBESITY; NEURONS; FLY Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23769