Universität zu Köln

Verescakova, Hana, Ambrozova, Gabriela ORCID: 0000-0002-8172-9746, Kubala, Lukas ORCID: 0000-0002-7729-7338, Perecko, Tomas, Koudelka, Adolf ORCID: 0000-0002-9998-8383, Vasicek, Ondrej ORCID: 0000-0001-5892-0457, Rudolph, Tanja K., Klinke, Anna, Woodcock, Steven R., Freeman, Bruce A. and Pekarova, Michaela (2017). Nitro-oleic acid regulates growth factor-induced differentiation of bone marrow-derived macrophages. Free Radic. Biol. Med., 104. S. 10 - 20. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1873-4596

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Abstract

Many diseases accompanied by chronic inflammation are connected with dysregulated activation of macrophage subpopulations. Recently, we reported that nitro-fatty acids (NO2-FAs), products of metabolic and inflammatory reactions of nitric oxide and nitrite, modulate macrophage and other immune cell functions. Bone marrow cell suspensions were isolated from mice and supplemented with macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF) in combination with NO2-OA for different times. RAW 264.7 macrophages were used for short-term (1-5 min) experiments. We discovered that NO2-OA reduces cell numbers, cell colony formation, and proliferation of macrophages differentiated with colony-stimulating factors (CSFs), all in the absence of toxicity. In a case of GM-CSF-induced bone marrow-derived macrophages (BMMs), NO2-OA acts via downregulation of signal transducer and activator of transcription 5 and extracellular signal-regulated kinase (ERK) activation. In the case of M-CSF-induced BMMs, NO2-OA decreases activation of M-CSFR and activation of related PI3K and ERR. Additionally, NO2-OA also attenuates activation of BMMs. In aggregate, we demonstrate that NO2-OA regulates the process of macrophage differentiation and that NO2-FAs represent a promising therapeutic tool in the treatment of inflammatory pathologies linked with increased accumulation of macrophages in inflamed tissues.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Verescakova, Hana UNSPECIFIED UNSPECIFIED UNSPECIFIED Ambrozova, Gabriela UNSPECIFIED orcid.org/0000-0002-8172-9746 UNSPECIFIED Kubala, Lukas UNSPECIFIED orcid.org/0000-0002-7729-7338 UNSPECIFIED Perecko, Tomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Koudelka, Adolf UNSPECIFIED orcid.org/0000-0002-9998-8383 UNSPECIFIED Vasicek, Ondrej UNSPECIFIED orcid.org/0000-0001-5892-0457 UNSPECIFIED Rudolph, Tanja K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Klinke, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Woodcock, Steven R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Freeman, Bruce A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pekarova, Michaela UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-238262
DOI:	10.1016/j.freeradbiomed.2017.01.003
Journal or Publication Title:	Free Radic. Biol. Med.
Volume:	104
Page Range:	S. 10 - 20
Date:	2017
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1873-4596
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COLONY-STIMULATING FACTOR; NITRATED FATTY-ACIDS; HEMATOPOIETIC STEM-CELLS; GM-CSF; TRANSCRIPTION FACTORS; MONOCYTE/MACROPHAGE DIFFERENTIATION; NITROLINOLEIC ACID; GENE-EXPRESSION; FACTOR-RECEPTOR; FACTOR-I Multiple languages Biochemistry & Molecular Biology; Endocrinology & Metabolism Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23826