Universität zu Köln

Soykan, Tolga, Kaempf, Natalie ORCID: 0000-0002-5466-3972, Sakaba, Takeshi, Vollweiter, Dennis, Goerdeler, Felix ORCID: 0000-0002-7190-9145, Puchkov, Dmytro ORCID: 0000-0001-8341-4847, Kononenko, Natalia L. and Haucke, Volker (2017). Synaptic Vesicle Endocytosis Occurs on Multiple Timescales and Is Mediated by Formin-Dependent Actin Assembly. Neuron, 93 (4). S. 854 - 871. CAMBRIDGE: CELL PRESS. ISSN 1097-4199

Full text not available from this repository.

Abstract

Neurotransmission is based on the exocytic fusion of synaptic vesicles (SVs) followed by endocytic membrane retrieval and the reformation of SVs. Recent data suggest that at physiological temperature SVs are internalized via clathrin-independent ultrafast endocytosis (UFE) within hundreds of milliseconds, while other studies have postulated a key role for clathrin-mediated endocytosis (CME) of SV proteins on a timescale of seconds to tens of seconds. Here we demonstrate using cultured hippocampal neurons as a model that at physiological temperature SV endocytosis occurs on several timescales from less than a second to several seconds, yet, is largely independent of clathrin. Clathrin-independent endocytosis (CIE) of SV membranes is mediated by actin-nucleating formins such as mDia1, which are required for the formation of presynaptic endosome-like vacuoles from which SVs reform. Our results resolve previous discrepancies in the field and suggest that SV membranes are predominantly retrieved via CIE mediated by formin-dependent actin assembly.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Soykan, Tolga UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaempf, Natalie UNSPECIFIED orcid.org/0000-0002-5466-3972 UNSPECIFIED Sakaba, Takeshi UNSPECIFIED UNSPECIFIED UNSPECIFIED Vollweiter, Dennis UNSPECIFIED UNSPECIFIED UNSPECIFIED Goerdeler, Felix UNSPECIFIED orcid.org/0000-0002-7190-9145 UNSPECIFIED Puchkov, Dmytro UNSPECIFIED orcid.org/0000-0001-8341-4847 UNSPECIFIED Kononenko, Natalia L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Haucke, Volker UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-239508
DOI:	10.1016/j.neuron.2017.02.011
Journal or Publication Title:	Neuron
Volume:	93
Number:	4
Page Range:	S. 854 - 871
Date:	2017
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1097-4199
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CLATHRIN-INDEPENDENT ENDOCYTOSIS; HIPPOCAMPAL SYNAPSES; MYOSIN-II; COMPENSATORY ENDOCYTOSIS; NEUROMUSCULAR-JUNCTION; MEMBRANE RETRIEVAL; CALCIUM-DEPENDENCE; NERVE-TERMINALS; LIVE CELLS; ENDOPHILIN Multiple languages Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23950