Kulkarni, Pranav and Frommolt, Peter ORCID: 0000-0002-1966-8014 (2017). Challenges in the Setup of Large-scale Next-Generation Sequencing Analysis Workflows. Comp. Struct. Biotechnol. J.., 15. S. 471 - 478. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 2001-0370

Full text not available from this repository.

Abstract

While Next-Generation Sequencing (NGS) can now be considered an established analysis technology for research applications across the life sciences, the analysis workflows still require substantial bioinformatics expertise. Typical challenges include the appropriate selection of analytical software tools, the speedup of the overall procedure using HPC parallelization and acceleration technology, the development of automation strategies, data storage solutions and finally the development of methods for full exploitation of the analysis results across multiple experimental conditions. Recently, NGS has begun to expand into clinical environments, where it facilitates diagnostics enabling personalized therapeutic approaches, but is also accompanied by new technological, legal and ethical challenges. There are probably as many overall concepts for the analysis of the data as there are academic research institutions. Among these concepts are, for instance, complex IT architectures developed in-house, ready-to-use technologies installed on-site as well as comprehensive Everything as a Service (XaaS) solutions. In this mini-review, we summarize the key points to consider in the setup of the analysis architectures, mostly for scientific rather than diagnostic purposes, and provide an overview of the current state of the art and challenges of the field. (C) 2017 Kulkarni, Frommolt. Published by Elsevier B.V. on behalf of the Research Network of Computational and Structural Biotechnology.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kulkarni, PranavUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Frommolt, PeterUNSPECIFIEDorcid.org/0000-0002-1966-8014UNSPECIFIED
URN: urn:nbn:de:hbz:38-244273
DOI: 10.1016/j.csbj.2017.10.001
Journal or Publication Title: Comp. Struct. Biotechnol. J..
Volume: 15
Page Range: S. 471 - 478
Date: 2017
Publisher: ELSEVIER SCIENCE BV
Place of Publication: AMSTERDAM
ISSN: 2001-0370
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CANCER; GENOMESMultiple languages
Biochemistry & Molecular Biology; Biotechnology & Applied MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/24427

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item