Universität zu Köln

Hinkelbein, Jochen ORCID: 0000-0003-3585-9459, Boehm, Lennert, Braunecker, Stefan, Adler, Christoph, De Robertis, Edoardo ORCID: 0000-0001-9182-6964 and Cirillo, Fabrizio (2017). Decreased Tissue COX5B Expression and Mitochondrial Dysfunction during Sepsis-Induced Kidney Injury in Rats. Oxidative Med. Cell. Longev., 2017. LONDON: HINDAWI LTD. ISSN 1942-0994

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Abstract

Background. Sepsis is defined as a life-threatening organ dysfunction due to a dysregulated host response to infection. Sepsis is the dominant cause of acute kidney injury (AKI), accounting for nearly 50% of episodes of acute renal failure. Signaling cascades and pathways within the kidney are largely unknown and analysis of these molecular mechanisms may enhance knowledge on pathophysiology and possible therapeutic options. Material and Methods. 26 male Wistar rats were assigned to either a sham group (control, N = 6) or sepsis group (N = 20; cecal ligature and puncture model, 24 and 48 hours after CLP). Surviving rats (n = 12) were decapitated at 24 hours (early phase; n = 6) or 48 hours (late phase; n = 6) after CLP and kidneys removed for proteomic analysis. 2D-DIGE and DeCyder 2D software (t-test, P < 0.01) were used for analysis of significantly regulated protein spots. MALDI-TOF in combination with peptide mass fingerprinting (PMF) as well as Western Blot analysis was used for protein identification. Bioinformatic network analyses (STRING, GeneMania, and PCViz) were used to describe protein-protein interactions. Results. 12 spots were identified with significantly altered proteins (P < 0.01) in the three analyzed groups. Two spots could not be identified. Four different proteins were found significantly changed among the groups: major urinary protein (MUP5), cytochrome c oxidase subunit B (COX5b), myosin-6 (MYH6), and myosin-7 (MYH7). A significant correlation with the proteins was found for mitochondrial energy production and electron transport. Conclusions. COX5B could be a promising biomarker candidate since a significant association was found during experimental sepsis in the present study. For future research, COX5B should be evaluated as a biomarker in both human urine and serum to identify sepsis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hinkelbein, Jochen UNSPECIFIED orcid.org/0000-0003-3585-9459 UNSPECIFIED Boehm, Lennert UNSPECIFIED UNSPECIFIED UNSPECIFIED Braunecker, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Adler, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED De Robertis, Edoardo UNSPECIFIED orcid.org/0000-0001-9182-6964 UNSPECIFIED Cirillo, Fabrizio UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-247507
DOI:	10.1155/2017/8498510
Journal or Publication Title:	Oxidative Med. Cell. Longev.
Volume:	2017
Date:	2017
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	1942-0994
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SEPTIC SHOCK; DIFFERENTIAL ALTERATIONS; NETWORK INTEGRATION; ANIMAL-MODELS; CYTOCHROME-C; OXYGEN; PROTEOMICS; GENES; MEDULLA; NEPHROTOXICITY Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24750