Universität zu Köln

Klein, Rebecca, Mahlberg, Nicolas, Ohren, Maurice, Ladwig, Anne, Neumaier, Bernd ORCID: 0000-0001-5425-3116, Graf, Rudolf, Hoehn, Mathias, Albrechtsen, Morten, Rees, Stephen, Fink, Gereon Rudolf, Rueger, Maria Adele and Schroeter, Michael (2016). The Neural Cell Adhesion Molecule-Derived (NCAM)-Peptide FG Loop (FGL) Mobilizes Endogenous Neural Stem Cells and Promotes Endogenous Regenerative Capacity after Stroke. J. Neuroimmune Pharm., 11 (4). S. 708 - 721. NEW YORK: SPRINGER. ISSN 1557-1904

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Abstract

The neural cell adhesion molecule (NCAM)-derived peptide FG loop (FGL) modulates synaptogenesis, neurogenesis, and stem cell proliferation, enhances cognitive capacities, and conveys neuroprotection after stroke. Here we investigated the effect of subcutaneously injected FGL on cellular compartments affected by degeneration and regeneration after stroke due to middle cerebral artery occlusion (MCAO), namely endogenous neural stem cells (NSC), oligodendrocytes, and microglia. In addition to immunohistochemistry, we used non-invasive positron emission tomography (PET) imaging with the tracer [F-18]-fluoro-L-thymidine ([F-18]FLT) to visualize endogenous NSC in vivo. FGL significantly increased endogenous NSC mobilization in the neurogenic niches as evidenced by in vivo and ex vivo methods, and it induced remyelination. Moreover, FGL affected neuroinflammation. Extending previous in vitro results, our data show that the NCAM mimetic peptide FGL mobilizes endogenous NSC after focal ischemia and enhances regeneration by amplifying remyelination and modulating neuroinflammation via affecting microglia. Results suggest FGL as a promising candidate to promote recovery after stroke.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klein, Rebecca UNSPECIFIED UNSPECIFIED UNSPECIFIED Mahlberg, Nicolas UNSPECIFIED UNSPECIFIED UNSPECIFIED Ohren, Maurice UNSPECIFIED UNSPECIFIED UNSPECIFIED Ladwig, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumaier, Bernd UNSPECIFIED orcid.org/0000-0001-5425-3116 UNSPECIFIED Graf, Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoehn, Mathias UNSPECIFIED UNSPECIFIED UNSPECIFIED Albrechtsen, Morten UNSPECIFIED UNSPECIFIED UNSPECIFIED Rees, Stephen UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Gereon Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Rueger, Maria Adele UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeter, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-254880
DOI:	10.1007/s11481-016-9694-5
Journal or Publication Title:	J. Neuroimmune Pharm.
Volume:	11
Number:	4
Page Range:	S. 708 - 721
Date:	2016
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1557-1904
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VIVO; DENTATE GYRUS; ADULT BRAIN; MICROGLIA/MACROPHAGE POLARIZATION; RECEPTOR AGONIST; ISCHEMIC-STROKE; MIMETIC PEPTIDE; M2 MICROGLIA; SPINAL-CORD; NCAM Multiple languages Neurosciences; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25488