Universität zu Köln

Schuler, Mara L., Mantegazza, Otho and Weber, Andreas P. M. (2016). Engineering C-4 photosynthesis into C-3 chassis in the synthetic biology age. Plant J., 87 (1). S. 51 - 66. HOBOKEN: WILEY. ISSN 1365-313X

Full text not available from this repository.

Abstract

C-4 photosynthetic plants outperform C-3 plants in hot and arid climates. By concentrating carbon dioxide around Rubisco C-4 plants drastically reduce photorespiration. The frequency with which plants evolved C-4 photosynthesis independently challenges researchers to unravel the genetic mechanisms underlying this convergent evolutionary switch. The conversion of C-3 crops, such as rice, towards C-4 photosynthesis is a long-standing goal. Nevertheless, at the present time, in the age of synthetic biology, this still remains a monumental task, partially because the C-4 carbon-concentrating biochemical cycle spans two cell types and thus requires specialized anatomy. Here we review the advances in understanding the molecular basis and the evolution of the C-4 trait, advances in the last decades that were driven by systems biology methods. In this review we emphasise essential genetic engineering tools needed to translate our theoretical knowledge into engineering approaches. With our current molecular understanding of the biochemical C-4 pathway, we propose a simplified rational engineering model exclusively built with known C-4 metabolic components. Moreover, we discuss an alternative approach to the progressing international engineering attempts that would combine targeted mutagenesis and directed evolution.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schuler, Mara L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mantegazza, Otho UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-271734
DOI:	10.1111/tpj.13155
Journal or Publication Title:	Plant J.
Volume:	87
Number:	1
Page Range:	S. 51 - 66
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AGROBACTERIUM-MEDIATED TRANSFORMATION; LIGHT-REGULATED EXPRESSION; BUNDLE-SHEATH; PHOSPHOENOLPYRUVATE CARBOXYKINASE; DICARBOXYLATE TRANSPORTERS; QUANTITATIVE PROTEOMICS; GENOME SEQUENCE; GENE-EXPRESSION; FOXTAIL MILLET; GLOBAL FOOD Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27173